Supplementary Materials

Supplementary Material for:

Coexistence of potent HIV-1 broadly neutralizing antibodies and antibody-sensitive viruses in a viremic controller

Natalia T. Freund, Haoqing Wang, Louise Scharf, Lilian Nogueira, Joshua A. Horwitz, Yotam Bar-On, Jovana Golijanin, Stuart A. Sievers, Devin Sok, Hui Cai, Julio C. Cesar Lorenzi, Ariel Halper-Stromberg, Ildiko Toth, Alicja Piechocka-Trocha, Harry B. Gristick, Marit J. van Gils, Rogier W. Sanders, Lai-Xi Wang, Michael S. Seaman, Dennis R. Burton, Anna Gazumyan, Bruce D. Walker, Anthony P. West Jr., Pamela J. Bjorkman, Michel C. Nussenzweig*

*Corresponding author. Email: nussen{at}rockefeller.edu

Published 18 January 2017, Sci. Transl. Med. 9, eaal2144 (2017)
DOI: 10.1126/scitranslmed.aal2144

This PDF file includes:

  • Methods
  • Fig. S1. Isolation of neutralizing antibody clones from donor EB354.
  • Fig. S2. Competitive ELISA.
  • Fig. S3. NC37 Fab–gp120 complex structure.
  • Fig. S4. Sequence comparison between BG18, PGT121, and 10-1074.
  • Fig. S5. BG18 antibody binding to synthetic HIV-1 V3 glycopeptides.
  • Fig. S6. env SGS of plasma virus from donor EB354.
  • Fig. S7. Viral loads in HIV1YU2–infected and BG18- and NC37-treated humanized mice.
  • Fig. S8. BG8 therapy in HIV1YU2–infected humanized mice.
  • Legends for tables S1 to S5
  • References (92104)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Clinical information, donor EB354 (provided in Excel).
  • Table S2 (Microsoft Excel format). IC50 and IC80 values in TZM-bl assay for antibodies NC37, BG1, BG18, BG8, and 10-1074 and PGT121 against a 118-virus panel (provided in Excel).
  • Table S3 (Microsoft Excel format). IC50 and IC80 values in TZM-bl assay of the 1:1:1 combination of NC37, BG1, and BG18 against a 118-virus panel (provided in Excel).
  • Table S4 (Microsoft Excel format). IC50 and IC80 values in TZM-bl assay of antibodies BG8, BG18, PGT121, and 12A12 against selected HIV-1 V3 envelope mutants (provided in Excel).
  • Table S5 (Microsoft Excel format). Crystallization statistics (provided in Excel).

[Download Tables S1 to S5]