Supplementary Materials

Supplementary Material for:

Pharmacological rescue of diabetic skeletal stem cell niches

Ruth Tevlin, Eun Young Seo, Owen Marecic, Adrian McArdle, Xinming Tong, Bryan Zimdahl, Andrey Malkovskiy, Rahul Sinha, Gunsagar Gulati, Xiyan Li, Taylor Wearda, Rachel Morganti, Michael Lopez, Ryan C. Ransom, Christopher R. Duldulao, Melanie Rodrigues, Allison Nguyen, Michael Januszyk, Zeshaan Maan, Kevin Paik, Kshemendra-Senarath Yapa, Jayakumar Rajadas, Derrick C. Wan, Geoffrey C. Gurtner, Michael Snyder, Philip A. Beachy, Fan Yang, Stuart B. Goodman, Irving L. Weissman, Charles K. F. Chan,* Michael T. Longaker*

*Corresponding author. Email: chazchan{at} (C.K.F.C.); longaker{at} (M.T.L.)

Published 11 January 2017, Sci. Transl. Med. 9, eaag2809 (2017)
DOI: 10.1126/scitranslmed.aag2809

This PDF file includes:

  • Materials and Methods
  • Fig. S1. MST apparatus and assessment.
  • Fig. S2. Impaired mSSC- and BCSP-mediated bone healing is consistent in multiple mouse models of DM.
  • Fig. S3. Resting glucose and weight between types of Db and pre-Db mouse models.
  • Fig. S4. Circulating IL-1b is elevated in both uninjured and injured Db mice.
  • Fig. S5. Local delivery of Ihh restores impaired bone healing in Db mice.
  • Fig. S6. Bone regeneration is impaired in Db versus WT mice, but osteoclastic activity is not notably affected.
  • Fig. S7. Neutralization of TNF╬▒ signaling in Db serum restores Ihh expression in cocultured mSSC.
  • Table S1. Primer sequence for qRT-PCR.
  • Reference (42)

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