Supplementary Materials

Supplementary Material for:

A PTK7-targeted antibody-drug conjugate reduces tumor-initiating cells and induces sustained tumor regressions

Marc Damelin,* Alexander Bankovich, Jeffrey Bernstein, Justin Lucas, Liang Chen, Samuel Williams, Albert Park, Jorge Aguilar, Elana Ernstoff, Manoj Charati, Russell Dushin, Monette Aujay, Christina Lee, Hanna Ramoth, Milly Milton, Johannes Hampl, Sasha Lazetic, Virginia Pulito, Edward Rosfjord, Yongliang Sun, Lindsay King, Frank Barletta, Alison Betts, Magali Guffroy, Hadi Falahatpisheh, Christopher J. O'Donnell, Robert Stull, Marybeth Pysz, Paul Escarpe, David Liu, Orit Foord, Hans Peter Gerber, Puja Sapra,* Scott J. Dylla*

*Corresponding author. Email: marc.damelin{at} (M.D.); puja.sapra{at} (P.S.); scott.dylla{at} (S.J.D.)

Published 11 January 2017, Sci. Transl. Med. 9, eaag2611 (2017)
DOI: 10.1126/scitranslmed.aag2611

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Immunohistochemistry of PTK7 in OVCA xenografts.
  • Fig. S2. Immunohistochemistry of PTK7 in NSCLC xenografts.
  • Fig. S3. Immunohistochemistry of PTK7 in TNBC xenografts.
  • Fig. S4. Immunohistochemistry of PTK7 in primary human tumors and normal human tissues.
  • Fig. S5. Characterization of the PTK7-targeted ADC.
  • Fig. S6. Characterization of the control ADC.
  • Fig. S7. Characterization of the OVCAR3 ovarian cancer model.
  • Fig. S8. Control treatments for mechanism of action studies.
  • Fig. S9. Examples of PTK7 expression in the stroma.
  • Fig. S10. Comparison of PTK7 immunohistochemistry with two antibodies.
  • Fig. S11. PTK7 expression on cultured human endothelial cells.
  • Fig. S12. PTK7 expression on dendritic cells in human whole blood.
  • Fig. S13. Summary of PTK7 staining on pDCs in primary NSCLC tumors.
  • Fig. S14. Histopathology from the monkey toxicology study.
  • Fig. S15. Monkey toxicokinetics.
  • Table S1. PTK7 expression in normal human tissues.
  • Table S2. Characterization of PDX tumor models.
  • Table S3. Toxicokinetics in monkey.

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