Supplementary Materials

Supplementary Material for:

A bioengineered living cell construct activates an acute wound healing response in venous leg ulcers

Rivka C. Stone, Olivera Stojadinovic, Ashley M. Rosa, Horacio A. Ramirez, Evangelos Badiavas, Miroslav Blumenberg, Marjana Tomic-Canic*

*Corresponding author. Email: mtcanic{at}med.miami.edu

Published 4 January 2017, Sci. Transl. Med. 9, eaaf8611 (2017)
DOI: 10.1126/scitranslmed.aaf8611

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Expression heat maps of top BLCC-regulated genes in clinical trial subjects.
  • Fig. S2. Networks of growth factors and their known effects on gene targets that are also regulated in chronic VLUs after BLCC application.
  • Fig. S3. Clinical trajectories of VLU study subjects.
  • Fig. S4. Subject-specific expression of cytokines.
  • Fig. S5. Posttreatment healing trajectories.
  • Table S1. Subject disposition.
  • Table S2. Exclusions from analysis of primary outcome.
  • Legend for table S3
  • Table S4. Genes corresponding to the top 10 enriched pathways after BLCC treatment.
  • Table S5. Evidence for TGFB1 activation post-BLCC treatment.
  • Table S6. Establishment of reference gene expression profiles for acute and chronic VLU wound healing.
  • Legends for tables S7 to S9
  • Table S10. Genes correlating with the healing trajectory in VLUs after BLCC treatment.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). Significantly regulated entities in the BLCC and control treatment groups.
  • Table S7 (Microsoft Excel format). Significantly regulated entities in the acute and chronic wound reference profiles.
  • Table S8 (Microsoft Excel format). Regulated genes corresponding to enriched pathways shared by acute wounds and BLCC-treated VLUs.
  • Table S9 (Microsoft Excel format). Regulated genes corresponding to enriched biological processes shared by acute wounds and BLCC-treated VLUs.