Supplementary Materials

Supplementary Material for:

The protein phosphatase 2A regulatory subunit PR70 is a gonosomal melanoma tumor suppressor gene

Léon C. L. van Kempen, Margaret Redpath, Mounib Elchebly, Kathleen Oros Klein, Andreas I. Papadakis, James S. Wilmott, Richard A. Scolyer, Per-Henrik Edqvist, Fredrik Pontén, Dirk Schadendorf, Anke F. van Rijk, Stefan Michiels, Anne Dumay, Anne Helbling-Leclerc, Philippe Dessen, Jasper Wouters, Marguerite Stass, Celia M. T. Greenwood, Ghanem E. Ghanem, Joost van den Oord, Jean Feunteun, Alan Spatz*

*Corresponding author. Email: alan.spatz{at}

Published 14 December 2016, Sci. Transl. Med. 8, 369ra177 (2016)
DOI: 10.1126/scitranslmed.aai9188

This PDF file includes:

  • Table S1. Patient and sample characteristics.
  • Fig. S1. Clustering of aCGH profiles.
  • Fig. S2. PPP2R3B and PR70 expression in melanoma.
  • Fig. S3. Schematic presentation of newly identified SNVs in PPP2R3B.
  • Fig. S4. Effect of PR70 expression on MM117 melanoma cells.
  • Fig. S5. Effect of PR70 down-regulation on cell morphology.
  • Fig. S6. PR70 expression decreased tumorigenicity of MM117 melanoma cells in mice.
  • Fig. S7. Decreasing PR70 expression in MM57 melanoma cells increased their in vivo growth capacity.
  • Fig. S8. Schematic presentation of the firing of origins of replications.
  • Fig. S9. Flow cytometry cell proliferation data following the release after a nocodazole arrest of MM117 cells with low (MM117ctrl) or high (MM117PPP2R3B) PR70 expression.
  • Fig. S10. PR70 dephosphorylates pRb.

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