Supplementary Materials

Supplementary Material for:

Aggregatibacter actinomycetemcomitans–induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid arthritis

Maximilian F. Konig, Loreto Abusleme, Jesper Reinholdt, Robert J. Palmer, Ricardo P. Teles, Kevon Sampson, Antony Rosen, Peter A. Nigrovic, Jeremy Sokolove, Jon T. Giles, Niki M. Moutsopoulos, Felipe Andrade*

*Corresponding author. Email: andrade{at}

Published 14 December 2016, Sci. Transl. Med. 8, 369ra176 (2016)
DOI: 10.1126/scitranslmed.aaj1921

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Protein citrullination is only detected in neutrophils co-incubated with Aa strains.
  • Fig. S2. LtxA induces neutrophil hypercitrullination in human saliva and across a range of calcium concentrations.
  • Fig. S3. Antibodies against LtxA and Aa serotype b identify patients with active periodontitis associated with leukotoxic strains of Aa.
  • Fig. S4. Anti-LtxA antibody concentrations correlate with periodontitis severity.
  • Fig. S5. Antibodies to P. gingivalis are enriched in a subset of RA that largely differs from patients exposed to LtxA.
  • Table S1. Specific bacterial peptides identified in GCF.
  • Legends for tables S2 and S3
  • Table S4. Characteristics of healthy controls without periodontitis and patients with chronic periodontitis.
  • Table S5. Characteristics of RA patients by anti-LtxA antibody status.
  • Table S6. The association of SE alleles with ACPAs and RF according to anti–P. gingivalis antibody status.
  • References (6264)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Citrullinated peptides identified in untreated PMNs.
  • Table S3 (Microsoft Excel format). Citrullinated peptides identified in LtxA-treated PMNs.

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