Supplementary Materials

Supplementary Material for:

Five-coordinate H64Q neuroglobin as a ligand-trap antidote for carbon monoxide poisoning

Ivan Azarov, Ling Wang, Jason J. Rose, Qinzi Xu, Xueyin N. Huang, Andrea Belanger, Ying Wang, Lanping Guo, Chen Liu, Kamil B. Ucer, Charles F. McTiernan, Christopher P. O'Donnell, Sruti Shiva, Jesús Tejero, Daniel B. Kim-Shapiro, Mark T. Gladwin*

*Corresponding author. Email: gladwinmt{at}upmc.edu

Published 7 December 2016, Sci. Transl. Med. 8, 368ra173 (2016)
DOI: 10.1126/scitranslmed.aah6571

This PDF file includes:

  • Methods
  • Fig. S1. Example measurement of P50 of oxygen of Ngb-H64Q.
  • Fig. S2. Oxygen binding and dissociation kinetics.
  • Fig. S3. Autoxidation of Ngb-H64Q-CCC in aerobic conditions.
  • Fig. S4. Nitrite reduction by Ngb-H64Q-CCC in the presence of sodium dithionite.
  • Fig. S5. Standard absorbance reference spectra at 37°C.
  • Fig. S6. Dissociation of CO from Hb at room temperature in the presence of excess Ngb-H64Q-CCC and NO.
  • Fig. S7. Clearance of Ngb H64Q-CCC in plasma in a lethal CO poisoning model.
  • Fig. S8. Ngb-H64Q-CCC safety and toxicity data after 48 hours of treatment with Ngb-H64Q-CCC.
  • Table S1. Binding parameters for oxygen and CO and autoxidation rates of selected CO binding molecules.
  • Table S2. Measured redox status of infused Ngb-H64Q-CCC and CO-Hb decreases at 5 min after CO stops for the mice in nonlethal CO poisoning model (n = 6).
  • References (6871)

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