Supplementary Materials

Supplementary Material for:

Imaging mitochondrial dynamics in human skin reveals depth-dependent hypoxia and malignant potential for diagnosis

Dimitra Pouli, Mihaela Balu, Carlo A. Alonzo, Zhiyi Liu, Kyle P. Quinn, Francisca Rius-Diaz, Ronald M. Harris, Kristen M. Kelly, Bruce J. Tromberg, Irene Georgakoudi*

*Corresponding author. Email: irene.georgakoudi{at}tufts.edu

Published 30 November 2016, Sci. Transl. Med. 8, 367ra169 (2016)
DOI: 10.1126/scitranslmed.aag2202

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Automated image analysis steps before quantitative extraction of cellular-related biomarkers.
  • Fig. S2. Setup for dynamic media oxygen saturation and live oxygen saturation measurements.
  • Fig. S3. Automated digital filter–based identification of endogenous TPEF contributions from non-NADH chromophores.
  • Fig. S4. Detailed mitochondrial clustering profiles.
  • Fig. S5. The depth-dependent mean PSD variance is correlated with the corresponding N/C ratio (nuclear-to-cytoplasmic ratio) variation.
  • Fig. S6. Organization patterns in stained mitochondria images of 2D cultures of undifferentiated, differentiated, and apoptotic cells.
  • Fig. S7. Mitochondrial organization distinguishes benign from malignant macroscopically suspicious sites.

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