Supplementary Materials

Supplementary Material for:

Oral thrombin inhibitor aggravates platelet adhesion and aggregation during arterial thrombosis

Tobias Petzold,* Manuela Thienel, Ildiko Konrad, Irene Schubert, Ron Regenauer, Boj Hoppe, Michael Lorenz, Annekathrin Eckart, Sue Chandraratne, Carsten Lennerz, Christof Kolb, Daniel Braun, Janina Jamasbi, Richard Brandl, Siegmund Braun, Wolfgang Siess, Christian Schulz, Steffen Massberg

*Corresponding author. Email: tobias.petzold{at}med.uni-muenchen.de

Published 30 November 2016, Sci. Transl. Med. 8, 367ra168 (2016)
DOI: 10.1126/scitranslmed.aad6712

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Platelet reactivity is not altered under OTI compared to VKA treatment and nonanticoagulated blood.
  • Fig. S2. Adhesion size categories are characterized by area size and thrombus volume.
  • Fig. S3. Prothrombotic effects of OTI are found under CTI anticoagulation and are independent from OTI plasma concentration.
  • Fig. S4. Inhibition of GPIbα-vWF interaction reduces thrombus formation on plaque material under arterial flow conditions.
  • Fig. S5. IL-4R/GPIbα-tg chimeras have normal platelet counts and lack GPIbα surface expression.
  • Fig. S6. Platelet adhesion and thrombus formation depend on GPIbα-thrombin interaction but not catalytically active thrombin.
  • Table S1. Hematological and clinical baseline characteristics are similar between treatment groups.
  • Legends for movies S1 to S5
  • Reference (51)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Flow chamber experiment on a plaque homogenate.
  • Movie S2 (.avi format). Confocal microscopy 3D reconstructions of flow chamber experiments.
  • Movie S3 (.avi format). Flow chamber experiment using CTI-inhibited blood.
  • Movie S4 (.avi format). Flow chamber experiment on the plaque homogenate after aspirin treatment.
  • Movie S5 (.avi format). Flow chamber experiment in the presence of VM16D GPIbα–blocking antibody.