Supplementary Materials

Supplementary Material for:

Effects of a gut pathobiont in a gnotobiotic mouse model of childhood undernutrition

Vitas E. Wagner, Neelendu Dey, Janaki Guruge, Ansel Hsiao, Philip P. Ahern, Nicholas P. Semenkovich, Laura V. Blanton, Jiye Cheng, Nicholas Griffin, Thaddeus S. Stappenbeck, Olga Ilkayeva, Christopher B. Newgard, William Petri, Rashidul Haque, Tahmeed Ahmed, Jeffrey I. Gordon*

*Corresponding author. Email: jgordon{at}wustl.edu

Published 23 November 2016, Sci. Transl. Med. 8, 366ra164 (2016)
DOI: 10.1126/scitranslmed.aah4669

This PDF file includes:

  • Fig. S1. Experimental designs.
  • Fig. S2. Generation of a Δbft-3 strain of ETBF.
  • Fig. S3. Assessing the effects of ETBF on gut barrier/immune function.
  • Legends for tables S1 to S8

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Summary of anthropometric scores and results of PCR assays for bft in the fecal microbiota of members of a birth cohort
    of 100 Bangladeshi children.
  • Table S2 (Microsoft Excel format). Composition and nutritional content of Bangladeshi diet embodiments, and nutritional requirements of mice and children.
  • Table S3 (Microsoft Excel format). V4-16S rRNA, COPRO-Seq, microbial RNA-seq, and cultured bacterial strain genome sequencing data sets.
  • Table S4 (Microsoft Excel format). Bacterial culture collection generated from the fecal microbiota of a severely stunted Bangladeshi child.
  • Table S5 (Microsoft Excel format). Genome features of isolates in the clonally arrayed bacterial culture collection generated from the fecal microbiota of a severely stunted 2-year-old Bangladeshi child.
  • Table S6 (Microsoft Excel format). Weights of all individual mice as a function of diet, microbiota, and dpg.
  • Table S7 (Microsoft Excel format). MS-based quantitation of metabolites in host tissues, serum, and cecum obtained from mice in the different treatment groups.
  • Table S8 (Microsoft Excel format). Effects of intraspecific and interspecific interactions on ETBF, NTBF, and other Bacteroides gene expression profiles in healthy or stunted donor community contexts.

[Download Tables S1 to S8]