Supplementary Materials

Supplementary Material for:

Lowered H3K27me3 and DNA hypomethylation define poorly prognostic pediatric posterior fossa ependymomas

Jill Bayliss, Piali Mukherjee, Chao Lu, Siddhant U. Jain, Chan Chung, Daniel Martinez, Benjamin Sabari, Ashley S. Margol, Pooja Panwalkar, Abhijit Parolia, Melike Pekmezci, Richard C. McEachin, Marcin Cieslik, Benita Tamrazi, Benjamin A. Garcia, Gaspare La Rocca, Mariarita Santi, Peter W. Lewis, Cynthia Hawkins, Ari Melnick, C. David Allis, Craig B. Thompson, Arul M. Chinnaiyan, Alexander R. Judkins,* Sriram Venneti*

*Corresponding author. Email: ajudkins{at}chla.usc.edu (A.R.J.); svenneti{at}med.umich.edu (S.V.)

Published 23 November 2016, Sci. Transl. Med. 8, 366ra161 (2016)
DOI: 10.1126/scitranslmed.aah6904

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Other histone modifications in pediatric PF ependymomas are not different.
  • Fig. S2. PF-ve tumors share similarities with PFA ependymomas.
  • Fig. S3. PF-ve tumors show lower DNA methylation compared to PF+ve ependymomas.
  • Fig. S4. CpGi methylation inversely correlates with H3K27me3 distribution.
  • Fig. S5. PFA/PF-ve ependymomas share epigenetic similarities with H3K27M gliomas.
  • Fig. S6. Radial glia in early cerebellar development show reduced H3K27me3.
  • Fig. S7. H3K9me3, H3K36me3, and H3K36me2 do not correlate with overall prognosis.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Comparison of PF-ve/PFA and PF+ve/PFB ependymomas.
  • Table S2 (Microsoft Excel format). Focused sequencing panel.
  • Table S3 (Microsoft Excel format). DAVID analysis of differentially methylated and regulated genes in PF-ve ependymomas.
  • Table S4 (Microsoft Excel format). Overlap of genes that exhibited common epigenetic alterations between H3K27M gliomas and PFA ependymomas with human radial glial signatures.
  • Table S5 (Microsoft Excel format). DAVID analysis of genes that exhibited common epigenetic alterations between H3K27M gliomas and PFA ependymomas.
  • Table S6 (Microsoft Excel format). Characteristics of human postmortem brain samples.
  • Table S7 (Microsoft Excel format). Data values in tabular format.

[Download Tables S1 to S7]