Supplementary Materials

Supplementary Material for:

Elucidating the interplay between IgG-Fc valency and FcγR activation for the design of immune complex inhibitors

Daniel F. Ortiz, Jonathan C. Lansing, Laura Rutitzky, Elma Kurtagic, Thomas Prod'homme, Amit Choudhury, Nathaniel Washburn, Naveen Bhatnagar, Christopher Beneduce, Kimberly Holte, Robert Prenovitz, Matthew Child, Jason Killough, Steven Tyler, Julia Brown, Stephanie Nguyen, Inessa Schwab, Maurice Hains, Robin Meccariello, Lynn Markowitz, Jing Wang, Radouane Zouaoui, Allison Simpson, Birgit Schultes, Ishan Capila, Leona Ling, Falk Nimmerjahn, Anthony M. Manning, Carlos J. Bosques*

*Corresponding author. Email: cbosques{at}momentapharma.com cbosques@momentapharma.com

Published 16 November 2016, Sci. Transl. Med. 8, 365ra158 (2016)
DOI: 10.1126/scitranslmed.aaf9418

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Generation and characterization of FcM-related materials.
  • Fig. S2. Characterization of multivalent Fc constructs.
  • Fig. S3. Monocyte activation dose response of Fc constructs.
  • Fig. S4. Analytical characterization of Fc3Y.
  • Fig. S5. Binding of IgG1, a trimeric IgG1 IC, Fc, and Fc3Y to FcγRs.
  • Fig. S6. Comparison of Fc3Y binding to platelets and other FcγR-expressing cells.
  • Fig. S7. Lack of induction of FcγRIIb phosphorylation by Fc3Y in Daudi B cells.
  • Fig. S8. Lack of neutrophil activation by Fc3Y.
  • Fig. S9. Fc3Y binding to Raji cells and lack of induction of ADCC.
  • Fig. S10. Fc3Y inhibition of moDC activation.
  • Fig. S11. Role of FcγRs, B cells, and endogenous IgG in an ITP mouse model.
  • Fig. S12. Fc3Y inhibition of FcγRIV-dependent 6A6-IgG2a–mediated platelet depletion in an ITP mouse model.
  • Fig. S13. Pharmacokinetic analysis of Fc3Y.
  • Table S1. Relative binding of Fc oligomers to FcγRs.
  • Legend for table S2
  • Reference (57)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S2. Source data (provided as an Excel file).

[Download Table S2]