Supplementary Materials

Supplementary Material for:

Cardiac repair in guinea pigs with human engineered heart tissue from induced pluripotent stem cells

Florian Weinberger, Kaja Breckwoldt, Simon Pecha, Allen Kelly, Birgit Geertz, Jutta Starbatty, Timur Yorgan, Kai-Hung Cheng, Katrin Lessmann, Tomas Stolen, Marielle Scherrer-Crosbie, Godfrey Smith, Hermann Reichenspurner, Arne Hansen, Thomas Eschenhagen*

*Corresponding author. Email: t.eschenhagen{at}uke.de

Published 2 November 2016, Sci. Transl. Med. 8, 363ra148 (2016)
DOI: 10.1126/scitranslmed.aaf8781

This PDF file includes:

  • Methods
  • Fig. S1. Characterization of hiPSC-CMs.
  • Fig. S2. Characterization of human endothelial cells.
  • Fig. S3. Teratoma assay.
  • Fig. S4. Histological identification of hEHTs after transplantation.
  • Fig. S5. Immunophenotyping of hEHTs after transplantation.
  • Fig. S6. Biodistribution of human cells.
  • Fig. S7. Histological identification of GCamp5G hEHTs after transplantation.
  • Fig. S8. 2P imaging of di-4-ANEPPS.
  • Table S1. Antibodies used for immunohistochemistry.
  • Table S2. Primer sequences (5′→3′).
  • Table S3. Fractional area change.
  • Table S4. LV end-diastolic diameter.
  • Reference (34)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Contracting hiPSC-EHT after 3 weeks in culture.
  • Movie S2 (.wmv format). Micro-CT scan of an injured heart immediately after hEHT transplantation.
  • Movie S3 (.mp4 format). Three-dimensional reconstruction of micro-CT images of a guinea pig heart immediately after hEHT transplantation.
  • Movie S4 (.mp4 format). GCamp5G fluorescence in hEHT before transplantation.
  • Movie S5 (.mp4 format). GCamp5G fluorescence in hiPSC-CMs.