Supplementary Materials

Supplementary Material for:

Genetic interrogation of circulating multiple myeloma cells at single-cell resolution

Jens G. Lohr,* Sora Kim, Joshua Gould, Birgit Knoechel, Yotam Drier, Matthew J. Cotton, Daniel Gray, Nicole Birrer, Bang Wong, Gavin Ha, Cheng-Zhong Zhang, Guangwu Guo, Matthew Meyerson, Andrew J. Yee, Jesse S. Boehm, Noopur Raje, Todd R. Golub*

*Corresponding author. Email: golub{at}broadinstitute.org (T.R.G.); jlohr{at}partners.org (J.G.L.)

Published 2 November 2016, Sci. Transl. Med. 8, 363ra147 (2016)
DOI: 10.1126/scitranslmed.aac7037

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Single-cell fluorescence microscopy.
  • Fig. S2. Single-cell and bulk DNA sequencing of MM cells from BM.
  • Fig. S3. Comparison of numbers of MM cells with mutations in blood and BM.
  • Fig. S4. Allelic fraction of mutations in single cells from MM cell lines.
  • Fig. S5. Comparative marker selection analysis of CTCs from two individual MM patients.
  • Table S1. Clinical data of MM patients for whom single-cell sequencing was performed.
  • Table S2. Primer sequences used in single-cell targeted DNA sequencing.
  • Table S3. Targeted next-generation sequencing libraries of single-cell DNA.
  • Legends for tables S4 and S5
  • References (3847)

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Other Supplementary Material for this manuscript includes the following:

  • Table S4 (Microsoft Excel format). Summary of GSEA results for normal plasma cells.
  • Table S5 (Microsoft Excel format). Gene expression of MM CTCs and BM cells by single-cell RNA-seq.

[Download Tables S4 and S5]