Supplementary Materials

Supplementary Material for:

Senescence-associated secretory phenotype contributes to pathological angiogenesis in retinopathy

Malika Oubaha, Khalil Miloudi, Agnieszka Dejda, Vera Guber, Gaëlle Mawambo, Marie-Anne Germain, Guillaume Bourdel, Natalija Popovic, Flavio A. Rezende, Randal J. Kaufman, Frédérick A. Mallette*, Przemyslaw Sapieha*

*Corresponding author. Email: mike.sapieha{at}umontreal.ca (P.S.); fa.mallette{at}umontreal.ca (F.A.M.)

Published 26 October 2016, Sci. Transl. Med. 8, 362ra144 (2016)
DOI: 10.1126/scitranslmed.aaf9440

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Induction of retinal cellular senescence during OIR and in an STZ model of type 1 diabetes.
  • Fig. S2. Vascular coverage during OIR.
  • Fig. S3. SEMA3A is secreted by senescent cells and triggers paracrine senescence in human retinal microvasculature endothelial cells.
  • Fig. S4. SEMA3A induces senescence in J774 macrophage/monocytes.
  • Fig. S5. Metformin inhibits senescence during OIR.
  • Fig. S6. Genes unaffected by metformin and aflibercept in OIR.
  • Fig. S7. Model for the quantification of retinal SA-β-gal.
  • Table S1. Antibodies.
  • Table S2. Primers.

[Download PDF]