Supplementary Materials

Supplementary Material for:

Hookworm recombinant protein promotes regulatory T cell responses that suppress experimental asthma

Severine Navarro*, Darren A. Pickering, Ivana B. Ferreira, Linda Jones, Stephanie Ryan, Sally Troy, Andrew Leech, Peter J. Hotez, Bin Zhan, Thewarach Laha, Roger Prentice, Tim Sparwasser, John Croese, Christian R. Engwerda, John W. Upham, Valerie Julia, Paul R. Giacomin, Alex Loukas*

*Corresponding author. Email: severine.navarro{at}jcu.edu.au (S.N.); alex.loukas{at}jcu.edu.au (A. Loukas)

Published 26 October 2016, Sci. Transl. Med. 8, 362ra143 (2016)
DOI: 10.1126/scitranslmed.aaf8807

This PDF file includes:

  • Fig. S1. Lung cytokine profile of mice treated with AcES.
  • Fig. S2. Denaturation of Ac-AIP-2 (dAIP-2) restores OVA-induced airway inflammation.
  • Fig. S3. Protection against OVA-induced airway inflammation with nonglycosylated recombinant AIP-2Q48.
  • Fig. S4. Ac-AIP-2–induced protection does not require the activation of TLRs.
  • Fig. S5. Intraperitoneal administration of Ac-AIP-2 does not induce cellular infiltration at the site of injection.
  • Fig. S6. Unadjuvanted Ac-AIP-2 treatment does not induce specific antibody production or in vitro T cell proliferation.
  • Fig. S7. Ac-AIP-2 does not impair vaccination (adjuvanted)–induced TH1-type inflammation.
  • Fig. S8. Ac-AIP-2 treatment modulates the expression of activation markers on the surface of MLN DCs.
  • Fig. S9. Ac-AIP-2 increases the frequency and number of mucosal Tregs.
  • Fig. S10. Ac-AIP-2 is captured by human DCs and decreases the expression of activation molecules.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Source data (Excel).

[Download Table S1]