Supplementary Materials

Supplementary Material for:

Selective targeting of mutant adenomatous polyposis coli (APC) in colorectal cancer

Lu Zhang, Panayotis C. Theodoropoulos, Ugur Eskiocak, Wentian Wang, Young-Ah Moon, Bruce Posner, Noelle S. Williams, Woodring E. Wright, Sang Bum Kim, Deepak Nijhawan, Jef K. De Brabander, Jerry W. Shay*

*Corresponding author. Email: jerry.shay{at}utsouthwestern.edu

Published 19 October 2016, Sci. Transl. Med. 8, 361ra140 (2016)
DOI: 10.1126/scitranslmed.aaf8127

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Ectopic expression of APC truncation confers tumorigenic properties.
  • Fig. S2. Transient knockdown of APC truncation reduces cell proliferation and induces caspase 3/7 activation in CRC cells.
  • Fig. S3. Reduction of serum in the medium confers sensitivity of HCT116 cells to TASIN-1.
  • Fig. S4. TASIN-1 is selectively toxic toward cells with truncated APC.
  • Fig. S5. TASIN-1 induces apoptosis in vitro.
  • Fig. S6. TASIN-1 does not directly affect WNT pathway activity.
  • Fig. S7. TASIN-1 induces apoptosis in vivo.
  • Fig. S8. Long-term treatment with TASIN-1 does not induce overt toxicity in a genetically engineered CRC mouse model.
  • Fig. S9. Long-term treatment with TASIN-1 suppresses expression of inflammatory genes in vivo.
  • Fig. S10. TASIN-1 decreases intracellular cholesterol content in DLD1 but not in HCT116 cells.
  • Fig. S11. TASIN-1 decreases cholesterol synthesis rate to varying extents in CRC cells.
  • Fig. S12. TASIN-1 attenuates tumor burden in CPC;Apc mice fed a high-fat diet.
  • Fig. S13. Simvastatin exhibits less selectivity and potency toward truncated APC cells compared to TASIN-1.
  • Table S1. APC status and origin of non-CRC types.
  • Legend for table S2
  • Table S3. qPCR primer sets for SRE target genes.
  • Table S4. qPCR primer sets for inflammatory genes.
  • References (5860)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S2. Tumor volume measurements for xenograft experiments (provided as an Excel file).

[Download Table S2]