Supplementary Materials

Supplementary Material for:

Cancer cells induce metastasis-supporting neutrophil extracellular DNA traps

Juwon Park, Robert W. Wysocki, Zohreh Amoozgar, Laura Maiorino, Miriam R. Fein, Julie Jorns, Anne F. Schott, Yumi Kinugasa-Katayama, Youngseok Lee, Nam Hee Won, Elizabeth S. Nakasone, Stephen A. Hearn, Victoria Küttner, Jing Qiu, Ana S. Almeida, Naiara Perurena, Kai Kessenbrock , Michael S. Goldberg, Mikala Egeblad*

*Corresponding author. Email: egeblad{at}

Published 19 October 2016, Sci. Transl. Med. 8, 361ra138 (2016)
DOI: 10.1126/scitranslmed.aag1711

This PDF file includes:

  • Materials and Methods
  • Fig. S1. CXCL1 promotes neutrophil recruitment to tumors.
  • Fig. S2. NET formation is present in lungs of mice after injection of 4T1 cancer cells.
  • Fig. S3. Neutrophils were harvested for in vitro assay.
  • Fig. S4. G-CSF and p47phox promote NET formation and cancer cell invasion.
  • Fig. S5. Breast cancer cells induce the formation of lytic NETs.
  • Fig. S6. CM from neutrophils induced to undergo NET formation promotes invasion.
  • Fig. S7. Cancer cell–induced NETs do not promote extravasation.
  • Legend for movie S1
  • References (4349)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). NET-like structures form in vivo around 4T1 cancer cells in the lung.

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