Supplementary Materials

Supplementary Material for:

Hyperelastic "bone": A highly versatile, growth factor–free, osteoregenerative, scalable, and surgically friendly biomaterial

Adam E. Jakus, Alexandra L. Rutz, Sumanas W. Jordan, Abhishek Kannan, Sean M. Mitchell, Chawon Yun, Katie D. Koube, Sung C. Yoo, Herbert E. Whiteley, Claus-Peter Richter, Robert D. Galiano, Wellington K. Hsu, Stuart R. Stock, Erin L. Hsu, Ramille N. Shah*

*Corresponding author. Email: ramille-shah{at}

Published 28 September 2016, Sci. Transl. Med. 8, 358ra127 (2016)
DOI: 10.1126/scitranslmed.aaf7704

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Additional functionalities and potential applications of 3D-printed HB.
  • Fig. S2. Microstructure of 1:1 HA/PLGA hot-melt 3D-printed composite.
  • Fig. S3. Additional rheological and mechanical properties of HB.
  • Fig. S4. Origin of HB’s mechanical properties and additional mechanical data.
  • Fig. S5. Axial compressive loading of HB femoral section by hand.
  • Fig. S6. Additional in vitro results: hMSCs seeded onto 30° advancing angle HAPCL scaffolds.
  • Fig. S7. Additional mouse subcutaneous implant in vivo results 7 and 35 days after implantation.
  • Fig. S8. Additional in vivo SEM micrographs of HB scaffolds explanted 35 days after being subcutaneously implanted into a mouse.
  • Fig. S9. Contrast-enhanced grayscale (non–false-colored) version of Fig. 8 (L and M).
  • Legends for movies S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). A 32x speed movie of HAPLGA ink being 3D-printed into 14-cm-tall, 6-mm-diameter cylinder composed of hundreds of layers, followed by a 64x speed movie illustrating HAPLGA being 3D-printed into 7.5-cm-tall double helix modeled after the structure of DNA.
  • Movie S2 (.mp4 format). 3D printing and physical manipulation of HB (HAPLGA) sheets.
  • Movie S3 (.mp4 format). HA/PLGA (1:1) hot-melt 3D-printed object being impacted and shattered by a hammer, followed by 3D-printed HAPLGA undergoing a series of hammer impacts and bouncing back.
  • Movie S4 (.mp4 format). Longitudinal compression, axial cyclic, and finger compression of hydrated 3D-printed HB femoral midshafts shown in Fig. 3.
  • Movie S5 (.mp4 format). Synchrotron microCT of 8-week PLF-explanted HAPLGA (HB) scaffolds (black and white speckled object in the movie), without and with 3 μg of rhBMP-2 added, illustrating new bone (dense white) material within and around HB.