Supplementary Materials

Supplementary Material for:

Inhibition of ileal bile acid uptake protects against nonalcoholic fatty liver disease in high-fat diet–fed mice

Anuradha Rao, Astrid Kosters, Jamie E. Mells, Wujuan Zhang, Kenneth D. R. Setchell, Angelica M. Amanso, Grace M. Wynn, Tianlei Xu, Brad T. Keller, Hong Yin, Sophia Banton, Dean P. Jones, Hao Wu, Paul A. Dawson, Saul J. Karpen*

*Corresponding author. Email: skarpen{at}emory.edu

Published 21 September 2016, Sci. Transl. Med. 8, 357ra122 (2016)
DOI: 10.1126/scitranslmed.aaf4823

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Inhibition of ileal BA absorption does not affect intestinal and colonic gene expression, food and water consumption, and hepatic injury markers.
  • Fig. S2. Inhibition of ileal BA absorption improves whole-body insulin tolerance and hepatic cholesterol concentration.
  • Fig. S3. Inhibition of ileal BA absorption does not affect hepatic fibrosis.
  • Fig. S4. Inhibition of ileal BA absorption changed hepatic BA composition and correlated with TG and cholesterol concentrations.
  • Fig. S5. Inhibition of ileal BA absorption changed global hepatic gene expression during 16 weeks of HFD feeding.
  • Fig. S6. Inhibition of ileal BA absorption changed gene expression in the mouse liver.
  • Fig. S7. Inhibition of ileal BA absorption does not affect hepatic and ileal ceramide amount and composition.
  • Fig. S8. Inhibition of ileal BA absorption affects hepatic gene expression in WT and Asbt−/− mice fed with chow or HFD for 1 week.
  • Legend for table S1
  • Table S2. Individual elements of NAS.
  • Table S3. Composite BA mixtures of six BAs representing the major in vivo hepatic BA species in HFD and HFD/ASBTi16w mice.
  • Legend for table S4
  • Table S5. HFD composition and macronutrient information.
  • Table S6. Primer sequences used for real-time PCR analysis.
  • Legend for table S7
  • References (4253)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S1. P values for all comparisons (provided as an Excel file).
  • Table S4. Differentially expressed genes and pathway analysis (provided as an Excel file).
  • Table S7. Primary data (provided as an Excel file).

[Download Tables S1, S4, and S7]