Supplementary Materials

Supplementary Material for:

Enhanced T cell responses to IL-6 in type 1 diabetes are associated with early clinical disease and increased IL-6 receptor expression

Christian Hundhausen, Alena Roth, Elizabeth Whalen, Janice Chen, Anya Schneider, S. Alice Long, Shan Wei, Rebecca Rawlings, MacKenzie Kinsman, Stephen P. Evanko, Thomas N. Wight, Carla J. Greenbaum, Karen Cerosaletti, Jane H. Buckner*

*Corresponding author. Email: jbuckner{at}

Published 14 September 2016, Sci. Transl. Med. 8, 356ra119 (2016)
DOI: 10.1126/scitranslmed.aad9943

This PDF file includes:

  • Fig. S1. Gating strategy for the analysis of IL-6/pSTAT3 by flow cytometry.
  • Fig. S2. Baseline pSTAT3.
  • Fig. S3. Reproducibility of phospho-flow assay and stability of the IL-6/pSTAT3 phenotype.
  • Fig. S4. Correlation of IL-6/pSTAT3 and IL-6/pSTAT1.
  • Fig. S5. IL-6R and ADAM17 cell surface staining.
  • Fig. S6. Correlation of mbIL-6R expression and IL-6/pSTAT3 in naïve and memory T cell subsets.
  • Fig. S7. Serum cytokine concentrations.
  • Fig. S8. Western blot for the ADAM17 protein in CD3 T cells.
  • Fig. S9. Functional role of ADAM17 in basal and activation-induced IL-6R shedding.
  • Fig. S10. Frequency and cytokine profile of peripheral blood Teff and Treg cells.
  • Fig. S11. RNA-seq: Overview of IL-6–regulated genes.
  • Fig. S12. qRT-PCR validation of RNA-seq data.
  • Fig. S13. Effect of IL-6 on surface expression of CCR7 and CD62L in CD8 T cells.
  • Fig. S14. Gating strategy for the analysis of proinflammatory chemokine receptors on TCM versus TEM cells.
  • Fig. S15. IL-6 promotes CD4 T cell migration.
  • Table S1. KEGG pathway mapping.
  • Table S2. KEGG pathway cytokine–cytokine receptor interaction: IL-6–induced genes.
  • Table S3. Normalized counts of IL-6–regulated genes.
  • Table S4. GO enrichment analysis.
  • Table S5. GO terms associated with T cell motility: IL-6–induced genes.
  • Table S6. Study participant characteristics.

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