Supplementary Materials

Supplementary Material for:

Pitx2 modulates a Tbx5-dependent gene regulatory network to maintain atrial rhythm

Rangarajan D. Nadadur, Michael T. Broman, Bastiaan Boukens, Stefan R. Mazurek, Xinan Yang, Malou van den Boogaard, Jenna Bekeny, Margaret Gadek, Tarsha Ward, Min Zhang, Yun Qiao, James F. Martin, Christine E. Seidman, Jon Seidman, Vincent Christoffels, Igor R. Efimov, Elizabeth M. McNally, Christopher R. Weber, Ivan P. Moskowitz*

*Corresponding author. Email: imoskowitz{at}uchicago.edu

Published 31 August 2016, Sci. Transl. Med. 8, 354ra115 (2016)
DOI: 10.1126/scitranslmed.aaf4891

This PDF file includes:

  • Materials and Methods
  • Fig. S1. No change in cardiac function after onset of AF in Tbx5fl/fl;R26CreERt2 mice.
  • Fig. S2. Tbx5fl/fl;R26CreERt2 develop more severe ventricular arrhythmias over time.
  • Fig. S3. Tbx5fl/fl;R26CreERt2 show paroxysmal AF.
  • Fig. S4. Optical AP from right atrium of Tbx5fl/fl;R26CreERt2 mice.
  • Fig. S5. Atrial rhythm instability and AF inducibility in Tbx5fl/+;R26CreERt2 is rescued by Pitx2 haploinsufficiency.
  • Table S1. qRT-PCR primers used.
  • Legends for movies S1 to S3
  • References (3339)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Ex vivo optical mapping in Tbx5fl/fl;R26CreERt2 mice at 7 days after TM.
  • Movie S2 (.avi format). Ex vivo optical mapping in Tbx5fl/fl;R26CreERt2 mice at 12 days after TM.
  • Movie S3 (.avi format). Ex vivo optical mapping in Tbx5fl/fl;R26CreERt2 mice at 14 days after TM.

[Download Movies S1 to S3]