Supplementary Materials

Supplementary Material for:

Thymidine phosphorylase exerts complex effects on bone resorption and formation in myeloma

Huan Liu, Zhiqiang Liu, Juan Du, Jin He, Pei Lin, Behrang Amini, Michael W. Starbuck, Nora Novane, Jatin J. Shah, Richard E. Davis, Jian Hou, Robert F. Gagel, Jing Yang*

*Corresponding author. Email: jiyang{at}

Published 24 August 2016, Sci. Transl. Med. 8, 353ra113 (2016)
DOI: 10.1126/scitranslmed.aad8949

This PDF file includes:

  • Methods
  • Fig. S1. Modulation of TP expression does not affect the growth and survival of myeloma cells.
  • Fig. S2. Myeloma-expressed TP enhances RANKL-mediated osteoclast differentiation and activity in vitro.
  • Fig. S3. Myeloma-expressed TP enhances NFATc1 expression and activity through inhibition of IRF8.
  • Fig. S4. Myeloma-expressed TP inhibits osteoblast differentiation and activity in vitro.
  • Fig. S5. TP inhibits the expression and activities of RUNX2 and osterix in vitro.
  • Fig. S6. Myeloma-expressed TP enhances DNMT3A levels in MSCs and preOCs.
  • Fig. S7. Myeloma cells with high TP expression secrete more 2DDR, which affects osteoclast and osteoblast differentiation in vitro.
  • Fig. S8. 2DDR up-regulates DNMT3A through α5β1Vβ3-PI3K/Akt signaling pathways.
  • Fig. S9. Knockdown of integrins or Akt1/2 abrogates the effects of 2DDR on DNMT3A expression.
  • Table S1. Primers for reverse transcription PCR and quantitative PCR.
  • Table S2. Primers for chromatin immunoprecipitation PCR.
  • Table S3. Primers for MSP and BSP.

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