Supplementary Materials

Supplementary Material for:

Spatially selective depletion of tumor-associated regulatory T cells with near-infrared photoimmunotherapy

Kazuhide Sato, Noriko Sato, Biying Xu, Yuko Nakamura, Tadanobu Nagaya, Peter L. Choyke, Yoshinori Hasegawa, Hisataka Kobayashi*

*Corresponding author. Email: kobayash{at}mail.nih.gov

Published 17 August 2016, Sci. Transl. Med. 8, 352ra110 (2016)
DOI: 10.1126/scitranslmed.aaf6843

This PDF file includes:

  • Fig. S1. Anti-CD25-F(ab′)2 and control-F(ab′)2 are generated, characterized, and conjugated with IR700 dye.
  • Fig. S2. NIR-PIT with anti-CD25-F(ab′)2–IR700 induces necrosis of CD25-expressing cells in a NIR light dose–dependent manner.
  • Fig. S3. Tumor-infiltrating CD8 T and NK cells do not express CD25.
  • Fig. S4. Anti-CD25-F(ab′)2–IR700 does not bind to the tumor cells.
  • Fig. S5. Depletion of tumor-infiltrating CD4+CD25+Foxp3+ Tregs with local CD25-targeted NIR-PIT is NIR light dose–dependent.
  • Fig. S6. Tumor-infiltrating CD4+Foxp3+ cells are depleted by local CD25-targeted NIR-PIT.
  • Fig. S7. Local CD25-targeted NIR-PIT of an MC38-luc tumor induces regression of the tumor.
  • Fig. S8. Local CD25-targeted NIR-PIT induces regression of TRAMP-C2-luc flank tumors.
  • Fig. S9. Repeated local CD25-targeted NIR-PIT enables prolonged suppression of tumor growth.
  • Fig. S10. Repeated local CD25-targeted NIR-PIT induces up-regulation of CD25 on CD8 T and NK cells at each treatment.
  • Fig. S11. Local CD25-targeted NIR-PIT induces activation of tumor-infiltrating dendritic cells and other APCs.
  • Fig. S12. Local CD25-targeted NIR-PIT increases the number of granulocytes in treated tumors.
  • Fig. S13. NIR light irradiation of tumors induces negligible production of cytokines and chemokines.
  • Fig. S14. Local CD25-targeted NIR-PIT induces systemic and intratumoral cytokine storm.
  • Fig. S15. IFN-γ production by CD8 T and NK cells in the lungs is not detected 1 day after CD25-targeted NIR-PIT.
  • Fig. S16. CD25-targeted NIR-PIT reduces IR700 fluorescence of the treated tumor, but not that of the contralateral nonirradiated tumor.
  • Fig. S17. Local CD25-targeted NIR-PIT of the right dorsal tumor induces reduction of multiple tumors at distant sites.
  • Fig. S18. Local CD25-targeted NIR-PIT inhibits the growth of tumor challenged on the contralateral side.
  • Fig. S19. The concentrations of cytokines and chemokines in the contralateral nonirradiated tumor are increased after CD25-targeted NIR-PIT.
  • Fig. S20. Antitumor effect of local CD25-targeted NIR-PIT is at least partly IFN-γ–dependent.

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