Supplementary Materials

Supplementary Material for:

ABCC1 confers tissue-specific sensitivity to cortisol versus corticosterone: A rationale for safer glucocorticoid replacement therapy

Mark Nixon, Scott D. Mackenzie, Ashley I. Taylor, Natalie Z. M. Homer, Dawn E. Livingstone, Rabah Mouras, Ruth A. Morgan, Damian J. Mole, Roland H. Stimson, Rebecca M. Reynolds, Alistair P. D. Elfick, Ruth Andrew, Brian R. Walker*

*Corresponding author. Email: b.walker{at}ed.ac.uk

Published 17 August 2016, Sci. Transl. Med. 8, 352ra109 (2016)
DOI: 10.1126/scitranslmed.aaf9074

This PDF file includes:

  • Fig. S1. Whole gel PCR images of ABC transporter expression in tissues and cells.
  • Fig. S2. Correlation of ABCC1 mRNA and protein concentrations in human adipocytes.
  • Fig. S3. ABCC1 inhibition in SGBS adipocytes.
  • Fig. S4. Effects of ABCC1 inhibition on GR-mediated transcription.
  • Fig. S5. Optimization of CARS microscopy for detection of intracellular D8-corticosterone.
  • Table S1. Characteristics of lean and obese study participants providing adipose biopsy samples during surgery.
  • Table S2. Characteristics of Addison’s study participants.
  • Table S3. Summary of studies describing plasma corticosterone and cortisol concentrations in healthy subjects.
  • Table S4. Primer sequences for PCR and corresponding expected product size.
  • Table S5. Human primer sequences for qPCR and corresponding probe number from Roche UPL.
  • Table S6. Murine primer sequences for qPCR and corresponding probe number from Roche UPL.

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Other Supplementary Material for this manuscript includes the following:

  • Table S7. Summary table of exact P values (provided as an Excel file).

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