Supplementary Materials

Supplementary Material for:

Hematopoietic stem cell transplantation in immunocompetent hosts without radiation or chemotherapy

Akanksha Chhabra, Aaron M. Ring, Kipp Weiskopf, Peter John Schnorr, Sydney Gordon, Alan C. Le, Hye-Sook Kwon, Nan Guo Ring, Jens Volkmer, Po Yi Ho, Serena Tseng, Irving L. Weissman, Judith A. Shizuru*

*Corresponding author. Email: jshizuru{at}stanford.edu

Published 10 August 2016, Sci. Transl. Med. 8, 351ra105 (2016)
DOI: 10.1126/scitranslmed.aae0501

This PDF file includes:

  • Fig. S1. Expression of c-Kit during hematopoiesis.
  • Fig. S2. ACK2 treatment does not affect functional HSC levels in Fcer1g−/− mice.
  • Fig. S3. FcgRIIb and FcgRIII blocking antibody 2.4G2 treatment alone does not deplete effector cells in Rag2−/−−/− mice.
  • Fig. S4. Anti–c-Kit antibody and CD47 antagonism suppress peripheral blood counts and are dependent on FcR function.
  • Fig. S5. Bone marrow composition after various doses of radiation.
  • Fig. S6. Preconditioning with anti–c-Kit and CV1mb enables long-term multilineage congenic hematopoietic engraftment in immunocompetent mice 20 weeks after transplantation.
  • Fig. S7. Preconditioning with anti–c-Kit and CV1mb enables stable multilineage chimerism in peripheral blood.
  • Fig. S8. Comparison of conditioning by combined anti–c-Kit and CD47 blockade versus radiation.
  • Fig. S9. Anti–c-Kit antibody ACK2 combined with additional CD47-blocking reagents enhances depletion of functional HSC in immunocompetent mice.
  • Fig. S10. Anti–c-Kit antibody ACK2 combined with additional CD47-blocking reagents enables granulocyte and lymphoid chimerism in transplanted immunocompetent recipients.
  • Fig. S11. Transient depletion of RBC parameters is observed using the combination of ACK2 and anti-CD47 antibody.
  • Fig. S12. Preconditioning with anti–c-Kit and anti-CD47 in combination with T cell–depleting antibodies enables stable multilineage chimerism in peripheral blood in an mHC-mismatched model of HSC allotransplantation.
  • Fig. S13. Preconditioning with anti–c-Kit, anti-CD47, and T cell–depleting antibodies enables long-term multilineage chimerism in immunocompetent mice 24 weeks after transplantation in an mHC-mismatched model of HSC allotransplantation.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Source data.

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