Supplementary Material for:

Relationship between vaginal microbial dysbiosis, inflammation, and pregnancy outcomes in cervical cerclage

Lindsay M. Kindinger, David A. MacIntyre,* Yun S. Lee, Julian R. Marchesi, Ann Smith, Julie A. K. McDonald, Vasso Terzidou, Joanna R. Cook, Christoph Lees, Fidan Israfil-Bayli, Yazmin Faiza, Philip Toozs-Hobson, Mark Slack, Stefano Cacciatore, Elaine Holmes, Jeremy K. Nicholson, T. G. Teoh, Phillip R. Bennett*

*Corresponding author. Email: d.macintyre{at}imperial.ac.uk (D.A.M.); p.bennett{at}imperial.ac.uk (P.R.B.)

Published 3 August 2016, Sci. Transl. Med. 8, 350ra102 (2016)
DOI: 10.1126/scitranslmed.aag1026

This PDF file includes:

• Fig. S1. Linear mixed-effects modeling of retrospective outcome data.
• Fig. S2. Gestation at birth as a function of suture material used in the prospectively recruited cohort.
• Fig. S3. Ward’s linkage HCA of species sequence data.
• Fig. S4. Longitudinal assessment of vaginal bacterial community structure after suture insertion.
• Fig. S5. In vitro adherence assay of suture material with E. coli or L. jensenii.
• Fig. S6. Longitudinal comparison of bacterial genera increased after braided suture insertion.
• Fig. S7. qPCR assessment of A. vaginae and G. vaginalis at 4 weeks after cerclage.
• Fig. S8. V1-V3 hypervariable region sequence alignment against major vaginal Lactobacillus species.
• Table S1. Patient demographics for the retrospective study cohort.
• Table S2. Contributing confounders for nonviable pregnancy and PTB <37 weeks.
• Table S3. Bacterial genus classification according to time from cerclage.
• Table S4. Bacterial species classification into CSTs according to time from cerclage.
• Table S5. Species classification into normal, L. iners–dominant, intermediate, and severe dysbiosis according to time from cerclage.
• Table S6. Mean bacterial counts of A. vaginae and G. vaginalis before and 4 weeks after cerclage insertion, as assessed by qPCR.
• Table S7. Mean fold change in analyte expression detectable in cervicovaginal fluid before and after cerclage insertion.
• Table S8. DNA identity for the V1-V3 region used in the analysis for the four main lactobacilli in CSTs I, II, III, and V.

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