Supplementary Materials

Supplementary Material for:

Increased GVHD-related mortality with broad-spectrum antibiotic use after allogeneic hematopoietic stem cell transplantation in human patients and mice

Yusuke Shono,* Melissa D. Docampo, Jonathan U. Peled, Suelen M. Perobelli, Enrico Velardi, Jennifer J. Tsai, Ann E. Slingerland, Odette M. Smith, Lauren F. Young, Jyotsna Gupta, Sophia R. Lieberman, Hillary V. Jay, Katya F. Ahr, Kori A. Porosnicu Rodriguez, Ke Xu, Marco Calarfiore, Hendrik Poeck, Silvia Caballero, Sean M. Devlin, Franck Rapaport, Jarrod A. Dudakov, Alan M. Hanash, Boglarka Gyurkocza, George F. Murphy, Camilla Gomes, Chen Liu, Eli L. Moss, Shannon B. Falconer, Ami S. Bhatt, Ying Taur, Eric G. Pamer, Marcel R. M. van den Brink,* Robert R. Jenq*

*Corresponding author. Email: jenqr{at}mskcc.org (R.R.J.); shonoy{at}mskcc.org (Y.S.); vandenbm{at}mskcc.org (M.R.M.v.d.B)

Published 18 May 2016, Sci. Transl. Med. 8, 339ra71 (2016)
DOI: 10.1126/scitranslmed.aaf2311

This PDF file includes:

  • Fig. S1. Six representative clinical cases with time courses depicting fecal microbiota composition and administration of antibiotic treatments during the course of allo-HSCT.
  • Fig. S2. Recipients treated with imipenem-cilastatin exhibit enhanced histological GVHD on day 21.
  • Fig. S3. Flow cytometric analysis of colon tissues in recipients on day 21.
  • Fig. S4. Recipients treated with imipenem-cilastatin show increased production of IL-23 in the colon on day 21.
  • Fig. S5. Abundance of bacterial OTUs, clustered by 16S rRNA sequence similarity, in recipients treated with imipenem-cilastatin or aztreonam.
  • Fig. S6. Analysis of SCFAs in cecum stool specimens.
  • Fig. S7. LEfSe analysis of relative numbers of sequences assigned to KEGG ortholog gene pathways after metagenomic shotgun sequencing.
  • Table S1. Summary of day 21 analyses after allo-HSCT.

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