Supplementary Materials

Supplementary Material for:

Delayed globin synthesis leads to excess heme and the macrocytic anemia of Diamond Blackfan anemia and del(5q) myelodysplastic syndrome

Zhantao Yang, Siobán B. Keel, Akiko Shimamura, Li Liu, Aaron T. Gerds, Henry Y. Li, Brent L. Wood, Bart L. Scott, Janis L. Abkowitz*

*Corresponding author. Email: janabk{at}uw.edu

Published 11 May 2016, Sci. Transl. Med. 8, 338ra67 (2016)
DOI: 10.1126/scitranslmed.aaf3006

This PDF file includes:

  • Patient information
  • Fig. S1. The in vitro erythroid differentiation of normal human BMMNCs mimics in vivo differentiation.
  • Fig. S2. Heme availability is highest in early erythroid cells.
  • Fig. S3. Preferential selection during erythropoiesis.
  • Fig. S4. Sequential assessment of mRNA in marrow cultures from DBA patient 1 and del(5q) MDS patient 6.
  • Fig. S5. Hemopexin facilitates heme export through FLVCR to improve the erythroid differentiation of DBA patient 1’s marrow cells.
  • Fig. S6. Studies of MDS patients with RARS and RCMD.
  • Fig. S7. P53 activation, heme excess, and ferroptosis.
  • Fig. S8. Isotype controls and gating strategy for flow cytometry.
  • Fig. S9. Unmodified complete Western blots.
  • Table S1. Clinical data from the MDS patients.
  • Table S2. Probe and primer set for quantitative real-time PCR.
  • Table S3. Source data and statistical calculations for Fig. 3.
  • Table S4. Source data and statistical calculations for Fig. 4.
  • Reference (48)

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