Supplementary Materials

Supplementary Material for:

Initiation of immune tolerance–controlled HIV gp41 neutralizing B cell lineages

Ruijun Zhang, Laurent Verkoczy, Kevin Wiehe, S. Munir Alam, Nathan I. Nicely, Sampa Santra, Todd Bradley, Charles W. Pemble IV, Jinsong Zhang, Feng Gao, David C. Montefiori, Hilary Bouton-Verville, Garnett Kelsoe, Kevin Larimore, Phillip D. Greenberg, Robert Parks, Andrew Foulger, Jessica N. Peel, Kan Luo, Xiaozhi Lu, Ashley M. Trama, Nathan Vandergrift, Georgia D. Tomaras, Thomas B. Kepler, M. Anthony Moody, Hua-Xin Liao, Barton F. Haynes*

*Corresponding author. Email: barton.haynes{at}

Published 27 April 2016, Sci. Transl. Med. 8, 336ra62 (2016)
DOI: 10.1126/scitranslmed.aaf0618

This PDF file includes:

  • Fig. S1. Rhesus macaque immunization regimen, reactivity of serum and mucosal fluids, and neutralization of HIV isolates.
  • Fig. S2. B cell repertoires of macaques 584 and 585.
  • Fig. S3. Rhesus macaque clonal lineages using VH and VL genes similar to 2F5VH/VL.
  • Fig. S4. Neutralization by members of the DH570 clone.
  • Fig. S5. Amino acid and lipid insertion propensity differences within the three DH570 lineage members for which crystal structures were determined.
  • Fig. S6. Binding of DH570 lineage antibodies to MPER liposomes.
  • Fig. S7. Replicate images for Fig. 1A.
  • Fig. S8. Replicate images for Fig. 1E.
  • Fig. S9. Replicate plots for Fig. 5.
  • Fig. S10. Representative mouse flow cytometry gating strategies.
  • Fig. S11. Representative rhesus flow cytometry gating strategies.
  • Table S1. Neutralization of HIV isolates by DH573.
  • Table S2. Neutralization of HIV isolates by the DH570 clonal lineage.
  • Table S3. Characteristics of the DH570 clonal lineage isolated from rhesus macaque 585.
  • Table S4. Time course for HIV neutralization in the TZM-bl/FcγRI assay.
  • Table S5. Data collection and refinement statistics.
  • Table S6. Kinetic rates of DH570 lineage members from branch I and IV binding to MPER peptide-liposomes by BLI.
  • Table S7. CDR3 lipid insertion propensity for select DH570 lineage members.
  • Table S8. Statistical analysis summary for Fig. 1.

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