Supplementary Materials

Supplementary Material for:

IRF7 inhibition prevents destructive innate immunity—A target for nonantibiotic therapy of bacterial infections

Manoj Puthia, Ines Ambite, Caterina Cafaro, Daniel Butler, Yujing Huang, Nataliya Lutay, Gustav Rydström, Birgitta Gullstrand, Bhairavi Swaminathan, Aftab Nadeem, Björn Nilsson, Catharina Svanborg*

*Corresponding author. Email: catharina.svanborg{at}med.lu.se

Published 27 April 2016, Sci. Transl. Med. 8, 336ra59 (2016)
DOI: 10.1126/scitranslmed.aaf1156

This PDF file includes:

  • Fig. S1. CFT073-specific gene expression in infected human kidney cells.
  • Fig. S2. Bacterial persistence in Irf3−/−, Irf7−/−, and C57BL/6 mice infected with CFT073.
  • Fig. S3. Neutrophil recruitment in Irf3−/−, Irf7−/−, and C57BL/6 mice infected with CFT073.
  • Fig. S4. The ABU strain E. coli 83972 does not activate the IRF-7–driven hyperinflammatory response in mice.
  • Fig. S5. Pathology-associated activation of the granulocyte adhesion pathway genes in Irf3−/− mice.
  • Fig. S6. Activation of the CFT073-specific gene network in infected Irf3−/− mice.
  • Fig. S7. Control of mucosal expression of pathology-associated molecules in infected mice.
  • Fig. S8. IRF-3 and IRF-7 binding to CCL5 and IFNB1 promoter oligonucleotides.
  • Fig. S9. IRF7 promoter sequence.
  • Fig. S10. Primers used for amplification of the IRF7 promoter SNP rs3758650 (−886).
  • Fig. S11. Efficacy of Irf7 siRNA treatment in APN-susceptible Irf3−/− mice.
  • Fig. S12. Antibiotic treatment of APN-susceptible Irf3−/− mice.
  • Fig. S13. Staining and isotype controls.
  • Fig. S14. Unedited Western blot for fig. S8.
  • Fig. S15. Unedited gels for Fig. 4.
  • Fig. S16. Unedited gels for fig. S8.
  • Table S4. Association between the identified variants and IRF7 expression in the Icelandic population.
  • Table S5. Number of mice used in inoculation experiments.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Exome Aggregation Consortium IRF7.
  • Table S2. Exome Variant Server variant IRF7.
  • Table S3. Westra cis-eQTL IRF7.

[Download Tables S1 to S3]