Supplementary Materials

Supplementary Material for:

Amelioration of sepsis by TIE2 activation–induced vascular protect

Sangyeul Han,* Seung-Jun Lee, Kyung Eun Kim, Hyo Seon Lee, Nuri Oh, Inwon Park, Eun Ko, Seung Ja Oh, Yoon-Sook Lee, David Kim, Seungjoo Lee, Dae Hyun Lee, Kwang-Hoon Lee, Su Young Chae, Jung-Hoon Lee, Su-Jin Kim, Hyung-Chan Kim, Seokkyun Kim, Sung Hyun Kim,| Chungho Kim, Yoshikazu Nakaoka, Yulong He, Hellmut G. Augustin, Junhao Hu, Paul H. Song, Yong-In Kim, Pilhan Kim, Injune Kim, Gou Young Koh*

*Corresponding author. E-mail: gykoh{at}kaist.ac.kr (G.Y.K.); syhan69{at}gmail.com (S.H.)

Published 20 April 2016, Sci. Transl. Med. 8, 335ra55 (2016)
DOI: 10.1126/scitranslmed.aad9260

This PDF file includes:

  • Materials and Methods
  • Fig. S1. ABTAA binds to human and mouse ANG2.
  • Fig. S2. ABTAA activates TIE2 and its downstream effectors in human lung ECs.
  • Fig. S3. ABTAA stimulates internalization of TIE2.
  • Fig. S4. ABA and ABTAA have different effects on survival of wild-type and Ang1Δ/Δ septic mice in the pretreatment setting.
  • Fig. S5. Constitutive expression of ANG1 is critical for basal and ABTAA-induced TIE2 activity.
  • Fig. S6. Circulating ANG2 concentrations are very low in Ang2Δ/Δ mice.
  • Fig. S7. ABTAA has a longer half-life and is more effective than COMP-Ang1 for improving survival from sepsis.
  • Fig. S8. ABTAA protects against major organ injury and microvascular disintegration.
  • Fig. S9. Pretreatment with ABA or ABTAA mitigates parenchymal injuries and microvascular disintegration.
  • Fig. S10. Intravital microscopy was used to measure the pulmonary endothelial glycocalyx.
  • Fig. S11. ABTAA reduces pulmonary vascular leakage.
  • Fig. S12. Pretreatment with ABTAA blunts the cytokine storm in sepsis.
  • Fig. S13. Posttreatment with ABTAA does not attenuate cytokine storm in the primary endotoxemia model.
  • Fig. S14. Pretreatment with ABTAA blunts ANG2 surge in sepsis.
  • References (6070)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data for graphs.
  • Movie S1 (.mp4 format). Posttreatment with ABTAA rejuvenates septic mice.