Supplementary Materials

Supplementary Material for:

Skeletal muscle action of estrogen receptor α is critical for the maintenance of mitochondrial function and metabolic homeostasis in females

Vicent Ribas, Brian G. Drew, Zhenqi Zhou, Jennifer Phun, Nareg Y. Kalajian, Teo Soleymani, Pedram Daraei, Kevin Widjaja, Jonathan Wanagat, Thomas Q. de Aguiar Vallim, Amy H. Fluitt, Steven Bensinger, Thuc Le, Caius Radu, Julian P. Whitelegge, Simon W. Beaven, Peter Tontonoz, Aldons J. Lusis, Brian W. Parks, Laurent Vergnes, Karen Reue, Harpreet Singh, Jean C. Bopassa, Ligia Toro, Enrico Stefani, Matthew J. Watt, Simon Schenk, Thorbjorn Akerstrom, Meghan Kelly, Bente K. Pedersen, Sylvia C. Hewitt, Kenneth S. Korach, Andrea L. Hevener*

*Corresponding author. E-mail: ahevener{at}

Published 13 April 2016, Sci. Transl. Med. 8, 334ra54 (2016)
DOI: 10.1126/scitranslmed.aad3815

This PDF file includes:

  • Table S1. Basal metabolic characteristics in female subjects.
  • Table S2. Animal characteristics after a 6-hour fast in the basal or clamped state.
  • Table S3. Annotation of functionally enriched terms from Illumina microarray analyses on quadriceps muscle from female Control f/f versus MERKO mice.
  • Table S4. Microarray analyses on quadriceps muscle from Control f/f versus MERKO mice.
  • Table S5. Primer sequences for qPCR analyses on tissues and for genotyping of Control f/f and MERKO mice.
  • Fig. S1. Real-time respirometry in C2C12 myotubes.
  • Fig. S2. Muscle peak tension and time to fatigue.
  • Fig. S3. Polg1 expression is regulated by ERα in muscle.
  • Fig. S4. Altered mitochondrial fission-fusion signaling in ERα-deficient C2C12 myotubes.
  • Fig. S5. Calcineurin-PKA-Rcan1 axis in C2C12 myotubes with Esr1-KD.
  • Fig. S6. Expression of autophagy-related genes in MERKO muscle.
  • Fig. S7. Autophagic flux studies in C2C12 myocytes with Esr1-KD.

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