Supplementary Materials

Supplementary Material for:

NRF2 activation by antioxidant antidiabetic agents accelerates tumor metastasis

Hui Wang, Xiufei Liu, Min Long, Yi Huang, Linlin Zhang, Rui Zhang, Yi Zheng, Xiaoyu Liao, Yuren Wang, Qian Liao, Wenjie Li, Zili Tang, Qiang Tong, Xiaocui Wang, Fang Fang, Montserrat Rojo de la Vega, Qin Ouyang, Donna D. Zhang,* Shicang Yu,* Hongting Zheng*

*Corresponding author. E-mail: fnf7703{at}hotmail.com (H.Z.); yushicang{at}163.com (S.Y.); dzhang{at}pharmacy.arizona.edu (D.D.Z.)

Published 13 April 2016, Sci. Transl. Med. 8, 334ra51 (2016)
DOI: 10.1126/scitranslmed.aad6095

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Sax and Sit inhibit the activity of DPP-4.
  • Fig. S2. Sax and Sit do not affect tumor cell proliferation or sensitivity to chemotherapeutics.
  • Fig. S3. Sax, Sit, and Vil at different dosages accelerate tumor cell migration.
  • Fig. S4. Sax and Sit do not change the concentration of lactate and ATP production in tumor cells.
  • Fig. S5. The effect of Sax and Sit on GSH and GSSG concentrations.
  • Fig. S6. BSO prevents Sax- and Sit-induced cancer cell migration and invasion.
  • Fig. S7. Commercial forms of Sax and Sit activate NRF2 and promote tumor cell migration.
  • Fig. S8. Sax, Sit, Vil, Alo, and Lin do not share a common scaffold, but they all activate the NRF2 pathway.
  • Table S1. No increase in overall risk of tumors with DPP-4i monotherapy.
  • Table S2. No increase in overall risk of tumors with DPP-4i.
  • Table S3. No increased risk of digestive system tumors, skin tumors, or urinary system tumors with DPP-4i monotherapy.
  • Table S4. Clinical characteristics of NRF2 expression in liver cancer.
  • Table S5. Clinical characteristics of GCLM expression in liver cancer.
  • Table S6. The tissue origin and mutational profile of cancer cell lines used.
  • Table S8. List of primers and siRNA/shRNA sequences.
  • References (45113)

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Other Supplementary Material for this manuscript includes the following:

  • Table S7 Exact P values (provided as an Excel file).
  • Table S9 Original data (provided as an Excel file).