Supplementary Materials

Supplementary Material for:

Familial autoinflammation with neutrophilic dermatosis reveals a regulatory mechanism of pyrin activation

Seth L. Masters,* Vasiliki Lagou, Isabelle Jéru, Paul J. Baker, Lien Van Eyck, David A. Parry, Dylan Lawless, Dominic De Nardo, Josselyn E. Garcia-Perez, Laura F. Dagley, Caroline L. Holley, James Dooley, Fiona Moghaddas, Emanuela Pasciuto, Pierre-Yves Jeandel, Raf Sciot, Dena Lyras, Andrew I. Webb, Sandra E. Nicholson, Lien De Somer, Erika van Nieuwenhove, Julia Ruuth-Praz, Bruno Copin, Emmanuelle Cochet, Myrna Medlej-Hashim, Andre Megarbane, Kate Schroder, Sinisa Savic, An Goris, Serge Amselem, Carine Wouters,* Adrian Liston*

*Corresponding author. E-mail: masters{at} (S.L.M.); carine.wouters{at} (C.W.); adrian.liston{at} (A.L.)

Published 30 March 2016, Sci. Transl. Med. 8, 332ra45 (2016)
DOI: 10.1126/scitranslmed.aaf1471

This PDF file includes:

  • Fig. S1. Inflammatory manifestations of PAAND.
  • Fig. S2. Results from linkage analysis.
  • Fig. S3. Dominant inheritance of PAAND caused by mutation in pyrin, S242R.
  • Fig. S4. Inheritance of S242R mutations in additional pedigrees.
  • Fig. S5. Fluorescent microscopy of pyrin/ASC specks.
  • Fig. S6. Activation of caspase-1 and IL-1β by pyrin S242R.
  • Fig. S7. Proteomic analysis of pyrin interactions and phosphorylation.
  • Fig. S8. Functional effect of FMF mutations in context of pyrin S242R.
  • Fig. S9. Full Western blots.

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Other Supplementary Material for this manuscript includes the following: