Supplementary Materials

Supplementary Material for:

The tumor microenvironment and Immunoscore are critical determinants of dissemination to distant metastasis

Bernhard Mlecnik, Gabriela Bindea, Amos Kirilovsky, Helen K. Angell, Anna C. Obenauf, Marie Tosolini, Sarah E. Church, Pauline Maby, Angela Vasaturo, Mihaela Angelova, Tessa Fredriksen, Stéphanie Mauger, Maximilian Waldner,
Anne Berger, Michael R. Speicher, Franck Pagès, Viia Valge-Archer, Jérôme Galon*

*Corresponding author. E-mail: jerome.galon{at}

Published 24 February 2016, Sci. Transl. Med. 8, 327ra26 (2016)
DOI: 10.1126/scitranslmed.aad6352

This PDF file includes:

  • Fig. S1. Mutations of cancer genes in metastatic (M1) and nonmetastatic (M0) CRC patients.
  • Fig. S2. Genomic alterations in M0 and M1 CRC patients and their functional impact.
  • Fig. S3. Expression of genes known to be involved in metastasis, tumor progression, and WNT pathway in patients with (M1) or without (M0) metastasis.
  • Fig. S4. Immune gene expression in tumors from M0 and M1 patients.
  • Fig. S5. Blood and lymphatic vessels (BV, LV) in patients with (M1) or without (M0) metastasis.
  • Fig. S6. Intratumoral immune density in M0 and M1 patients.
  • Fig. S7. The impact of the tumor microenvironment components on metastasis.
  • Table S1. Clinical characteristic of the cohorts investigated.
  • Table S2. Forty-eight cancer-related genes tested for mutations shown in Fig. 1A.
  • Table S3. Association of central mutations in CRC and with clinical parameters.
  • Table S4. Fifteen cancer-related pathways shown in Fig. 1B.
  • Table S5. Amplified and deleted genes in M0 and M1 patients from cohorts 1 and 2.
  • Table S6. Genes involved in metastasis, tumor progression, and WNT pathway.

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