Supplementary Materials

Supplementary Material for:

TLR-7 activation enhances IL-22–mediated colonization resistance against vancomycin-resistant enterococcus

Michael C. Abt,* Charlie G. Buffie, Bože Sušac, Simone Becattini, Rebecca A. Carter, Ingrid Leiner, James W. Keith, David Artis, Lisa C. Osborne, Eric G. Pamer*

*Corresponding author. E-mail: abtm{at}mskcc.org (M.C.A.); pamere{at}mskcc.org (E.G.P.)

Published 24 February 2016, Sci. Transl. Med. 8, 327ra25 (2016)
DOI: 10.1126/scitranslmed.aad6663

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Norovirus infection is associated with reduced VRE colonization.
  • Fig. S2. R848 restores Reg3γ expression via TLR-7/MyD88 signaling on hematopoietic cells.
  • Fig. S3. ILCs are the dominant source of IL-22 after R848 treatment.
  • Fig. S4. Gating strategy for Figs. 3 and 4.
  • Fig. S5. R848 stimulates IL-22 expression in wild-type and Il1r1−/− ILCs.
  • Fig. S6. R848-driven induction of IL-22 and Reg3γ is dependent on TLR-7– expressing CD11c+ dendritic cells and IL-23 signaling.
  • Fig. S7. Full Western blot images from Figs. 2 (A to C) and 6D and figs. S2A and S5D.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Source data and exact P values.