Supplementary Materials

Supplementary Material for:

Long noncoding RNA Chast promotes cardiac remodeling

Janika Viereck, Regalla Kumarswamy, Ariana Foinquinos, Ke Xiao, Petros Avramopoulos, Meik Kunz, Marcus Dittrich, Tobias Maetzig, Karina Zimmer, Janet Remke, Annette Just, Jasmin Fendrich, Kristian Scherf, Emiliano Bolesani, Axel Schambach, Frank Weidemann, Robert Zweigerdt, Leon J. de Windt, Stefan Engelhardt, Thomas Dandekar, Sandor Batkai, Thomas Thum*

*Corresponding author. E-mail: thum.thomas{at}

Published 17 February 2016, Sci. Transl. Med. 8, 326ra22 (2016)
DOI: 10.1126/scitranslmed.aaf1475

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Chast is a lncRNA.
  • Fig. S2. Chast is located in all subcellular fractions of cardiomyocytes.
  • Fig. S3. Chast does not alter Arhgap27 levels.
  • Fig. S4. GapmeR-mediated silencing of Chast alters the transcriptome of HL-1 cells.
  • Fig. S5. Chast repression in vivo has no significant influence on plasma marker of kidney, neurological, or liver damage as well as on circulating proinflammatory IL-6 levels.
  • Table S1. Transcription factor binding site prediction for the Chast promoter.
  • Table S2. Cardiac relevant interaction partners enriched in the pulldown fraction of Chast-biotin compared to luciferase-biotin.
  • Table S3. OMIM enrichment analysis for the Chast-protein pulldown.
  • Table S4. Oligonucleotide primers used in this study.
  • References (3643)

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