Supplementary Materials

Supplementary Material for:

Timing of expression of the core clock gene Bmal1 influences its effects on aging and survival

Guangrui Yang, Lihong Chen, Gregory R. Grant, Georgios Paschos, Wen-Liang Song, Erik S. Musiek, Vivian Lee, Sarah C. McLoughlin, Tilo Grosser, George Cotsarelis, Garret A. FitzGerald*

*Corresponding author. E-mail: garret{at}

Published 3 February 2016, Sci. Transl. Med. 8, 324ra16 (2016)
DOI: 10.1126/scitranslmed.aad3305

This PDF file includes:

  • Fig. S1. Validation of Bmal1 deletion and dampening effect of other clock genes.
  • Fig. S2. Wheel-running activity, body weight, and fertility in cKO mice.
  • Fig. S3. Life span of iKO, cKO, and nKO mice.
  • Fig. S4. mRNA levels of Ccnd1 and Mki67 in skin.
  • Fig. S5. Other parameters in HFD-fed mice.
  • Fig. S6. iKO mice display similar Gfap induction in the brain as cKO.
  • Fig. S7. RNA-Seq results revealed dampening effect in core clock genes in iKO mice.
  • Table S1. Top 20 oscillating hepatic genes (JTK_CYCLE q value) in Ctrl mice show no circadian pattern in iKO mice.
  • Table S2. The ratio of differentially expressed gene numbers in cKO strain to the numbers in iKO strain.
  • Table S3. Summary of phenotypes of cKO and iKO mice.
  • Legends for data files S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Circadian transcriptome.
  • Data file S2 (Microsoft Excel format). Differentially expressed genes irrespective of time points.
  • Data file S3 (Microsoft Excel format). Phenotype enrichment analysis of differentially expressed genes.