Supplementary Materials

Supplementary Material for:

USF1 deficiency activates brown adipose tissue and improves cardiometabolic health

Pirkka-Pekka Laurila,* Jarkko Soronen, Sander Kooijman, Saara Forsström, Mariëtte R. Boon, Ida Surakka, Essi Kaiharju, Claudia P. Coomans, Sjoerd A. A. Van Den Berg, Anu Autio, Antti-Pekka Sarin, Johannes Kettunen, Emmi Tikkanen, Tuula Manninen, Jari Metso, Reija Silvennoinen, Krista Merikanto, Maija Ruuth, Julia Perttilä, Anne Mäkelä, Ayaka Isomi, Anita M. Tuomainen, Anna Tikka, Usama Abo Ramadan, Ilkka Seppälä, Terho Lehtimäki, Johan Eriksson, Aki Havulinna, Antti Jula, Pekka J. Karhunen, Veikko Salomaa, Markus Perola, Christian Ehnholm, Miriam Lee-Rueckert, Miranda Van Eck, Anne Roivainen, Marja-Riitta Taskinen, Leena Peltonen, Eero Mervaala, Anu Jalanko, Esa Hohtola, Vesa M. Olkkonen, Samuli Ripatti, Petri T. Kovanen, Patrick C. N. Rensen, Anu Suomalainen, Matti Jauhiainen*

*Corresponding author. E-mail: pirkka-pekka.laurila{at}thl.fi (P.-P.L.); matti.jauhiainen{at}thl.fi (M.J.)

Published 27 January 2016, Sci. Transl. Med. 8, 323ra13 (2016)
DOI: 10.1126/scitranslmed.aad0015

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Genotype distribution of offspring from Usf1+/− × Usf1+/− breeding.
  • Fig. S2. Serum lipid profiles in male Usf1−/− mice on HFD for 20 weeks.
  • Fig. S3. The clearance of [3H]oleate.
  • Fig. S4. Gene expression of Apoc2, Apoc3, Angptl3, and Apoa5 in liver.
  • Fig. S5. Adiposity of male Usf1−/− mice.
  • Fig. S6. Intestinal absorption of [3H]triolein.
  • Fig. S7. Intestinal absorption of [14C]oleate.
  • Fig. S8. Physical activity of USF1-deficient mice at thermoneutrality.
  • Fig. S9. Oxygen consumption and carbon dioxide production in weight-matched Usf1+/+ and Usf1+/− mice.
  • Fig. S10. Oxygen consumption and carbon dioxide production in Usf1+/+ and Usf1−/− mice at thermoneutrality.
  • Fig. S11. Thyroid hormone measurements in Usf1+/+ and Usf1−/− mice.
  • Fig. S12. Blood pressure levels in Usf1+/+ and Usf1−/− mice.
  • Fig. S13. Transmission electron micrographs of BAT from male mice.
  • Fig. S14. Organ distribution of [3H]oleate calculated per total tissue.
  • Fig. S15. Gene expression of fatty acid transporters in BAT in Usf1+/+ and Usf1−/− mice on HFD.
  • Fig. S16. Organ distribution of glycerol tri[3H]oleate calculated per total tissue.
  • Fig. S17. Ucp1 mRNA expression in BAT.
  • Fig. S18. Western blot images of UCP1 and respiratory chain complexes in BAT.
  • Fig. S19. Fur densities in Usf1−/− and Usf1+/+ mice. Fig. S20. Organ distribution of [14C]oleate at RT and thermoneutrality calculated per total tissue.
  • Fig. S21. Daily food intake of mice housed at thermoneutrality for 20 weeks.
  • Fig. S22. Correlation of weight gain and BAT uptake of [14C]oleate at both RT and thermoneutrality.
  • Fig. S23. Gene expression of adrenergic receptors in BAT in Usf1−/− and Usf1+/+ mice.
  • Fig. S24. Staining and quantitation of macrophages, smooth muscle cells, and collagen content in the aortic root lesion area from Usf1+/+Ldlr−/− and Usf−/−Ldlr−/− mice.
  • Fig. S25. Serum lipid profiles of Usf1+/+ Ldlr−/− and Usf1−/− Ldlr−/− mice on a HFD.
  • Fig. S26. Haploview representation of pairwise linkage disequilibria of the haploblock representative SNPs significantly associated with USF1 mRNA levels.
  • Fig. S27. SNPs within "Haploblock 1" as shown in table S3.
  • Fig. S28. SNPs within "Haploblock 2" as shown in table S3.
  • Fig. S29. SNPs within "Haploblock 3" as shown in table S3.
  • Fig. S30. SNPs within "Haploblock 4" as shown in table S3.
  • Fig. S31. SNPs within "Haploblock 5" as shown in table S3.
  • Table S1A. The lengths of USF1-deficient mice (between fig. S5 and fig. S6).
  • Table S1B. Fat percentage of USF1-deficient mice on a chow diet (between fig. S5 and fig. S6).
  • Table S2. Up-regulated and down-regulated pathways in Usf1−/− mouse BAT compared to Usf1+/+ mice (between fig. S18 and fig. S19).
  • Table S3. Association analysis of significant USF1 cis-eQTL haploblocks with metabolic phenotypes (between fig. S25 and fig. S26).
  • Table S4. Association analysis of SNPs within "Haploblock 1" with metabolic phenotypes.
  • Table S5. Association analysis of SNPs within "Haploblock 2" with metabolic phenotypes.
  • Table S6. Association analysis of SNPs within "Haploblock 3" with metabolic phenotypes.
  • Table S7. Association analysis of SNPs within "Haploblock 4" with metabolic phenotypes.
  • Table S8. Association analysis of SNPs within "Haploblock 5" with metabolic phenotypes.
  • References (52, 53)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Raw data (Excel)
  • Video S1(.mp4 format). BAT thermogenesis in response to norepinephrine injection in Usf1+/+ and Usf1+/− mice.