Supplementary Materials

Supplementary Material for:

Inhibition of diacylglycerol kinase α restores restimulation-induced cell death and reduces immunopathology in XLP-1

Elisa Ruffo, Valeria Malacarne, Sasha E. Larsen, Rupali Das, Laura Patrussi, Christoph Wülfing, Christoph Biskup, Senta M. Kapnick, Katherine Verbist, Paige Tedrick, Pamela L. Schwartzberg, Cosima T. Baldari, Ignacio Rubio, Kim E. Nichols, Andrew L. Snow, Gianluca Baldanzi, Andrea Graziani*

*Corresponding author. E-mail: graziani.andrea{at}hsr.it

Published 13 January 2016, Sci. Transl. Med. 8, 321ra7 (2016)
DOI: 10.1126/scitranslmed.aad1565

This PDF file includes:

  • Methods
  • Fig. S1. Activity of DGKα and DGKζ contributes to RICD resistance in T cells.
  • Fig. S2. DGKα silencing restores RICD in SAP-deficient cells through PKCθ- and Ras-mediated signaling pathways.
  • Fig. S3. DGKα blockade fails to rescue TCR-induced up-regulation of proapoptotic mediators FASL and BIM in SAP-deficient cells.
  • Fig. S4. Major steps of the automated segmentation and fluorescence quantification algorithm.
  • Fig. S5. DGKα inhibition enhances SAP-deficient CD8+ T cell cytotoxicity against autologous B cell targets.
  • Fig. S6. R59022 DGKα inhibitor does not impair viral clearance in the livers and spleens of LCMV infected Sh2d1a−⁄− mice.
  • Fig. S7. Gating strategies used in Figs. 7 and 8.
  • Table S1. Reagents.
  • Table S2. siRNA sequences.
  • Table S3. TaqMan gene expression arrays.
  • Table S4. Spot densitometry analysis for Western blotting.
  • Table S5. Statistical analyses.
  • Unmodified Western blot images.

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Other Supplementary Material for this manuscript includes the following: