Supplementary Materials

Supplementary Material for:

Virologic effects of broadly neutralizing antibody VRC01 administration during chronic HIV-1 infection

Rebecca M. Lynch, Eli Boritz, Emily E. Coates, Adam DeZure, Patrick Madden, Pamela Costner, Mary E. Enama, Sarah Plummer, Lasonji Holman, Cynthia S. Hendel, Ingelise Gordon, Joseph Casazza, Michelle Conan-Cibotti, Stephen A. Migueles, Randall Tressler, Robert T. Bailer, Adrian McDermott, Sandeep Narpala, Sijy O'Dell, Gideon Wolf, Jeffrey D. Lifson, Brandie A. Freemire, Robert J. Gorelick, Janardan P. Pandey, Sarumathi Mohan, Nicolas Chomont, Remi Fromentin, Tae-Wook Chun, Anthony S. Fauci, Richard M. Schwartz, Richard A. Koup, Daniel C. Douek, Zonghui Hu, Edmund Capparelli, Barney S. Graham, John R. Mascola,* Julie E. Ledgerwood,* VRC 601 Study Team

*Corresponding author. E-mail: jmascola{at}mail.nih.gov (J.R.M.); ledgerwood{at}mail.nih.gov (J.E.L.)

Published 23 December 2015, Sci. Transl. Med. 7, 319ra2061 (2015)
DOI: 10.1126/scitranslmed.aad5752

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Assessment of anti-VRC01 response after infusion with monoclonal VRC01.
  • Fig. S2. Characteristics of serum neutralization after VRC01 infusion.
  • Fig. S3. Population PK analysis of VRC01 infusion.
  • Fig. S4. Gating tree for flow cytometric sorting of cellular subsets from blood.
  • Fig. S5. Genetic diversity of preinfusion autologous virus in viremic subjects.
  • Fig. S6. The effect of VRC01 infusion on CD4 T cell–associated virus DNA levels in viremic individuals.
  • Fig. S7. Selection pressure on autologous virus from subject 20 after infusion with VRC01.
  • Fig. S8. Selection pressure on autologous virus from subject 21 after infusion with VRC01.
  • Fig. S9. Selection pressure on autologous virus from subject 24 after infusion with VRC01.
  • Fig. S10. Selection pressure on autologous virus from subject 25 after infusion with VRC01.
  • Fig. S11. Selection pressure on autologous virus from subject 23 after infusion with VRC01.
  • Fig. S12. Selection pressure on autologous virus from subject 26 after infusion with VRC01.
  • Fig. S13. Correlation of virus neutralization sensitivity to VRC01 and V5 loop length.
  • Fig. S14. Selection for reduced sensitivity to 3BNC117 in postinfusion virus.
  • Table S1. Clinical characteristics of HIV-1–infected subjects.
  • Table S2. Demographic characteristics of study participants.
  • Table S3. Reactogenicity after infusions with VRC01.
  • Table S4. VRC01 mean PK parameter values.
  • Table S5. Source data for cell-associated virus in aviremic subjects (Fig. 3).
  • Table S6. Characteristics of plasma virus kinetics in relation to serum antibody concentration.
  • Table S7. Sequence changes in Env protein sequences after infusion with VRC01.
  • Table S8. Sensitivity of pre- and postinfusion autologous virus clones from viremic subjects to mAbs.

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