Supplementary Materials

Supplementary Material for:

Tracking genetically engineered lymphocytes long-term reveals the dynamics of T cell immunological memory

Giacomo Oliveira, Eliana Ruggiero, Maria Teresa Lupo Stanghellini, Nicoletta Cieri, Mattia D'Agostino, Raffaele Fronza, Christina Lulay, Francesca Dionisio, Sara Mastaglio, Raffaella Greco, Jacopo Peccatori, Alessandro Aiuti, Alessandro Ambrosi, Luca Biasco, Attilio Bondanza, Antonio Lambiase, Catia Traversari, Luca Vago, Christof von Kalle, Manfred Schmidt, Claudio Bordignon, Fabio Ciceri, Chiara Bonini*

*Corresponding author. E-mail: bonini.chiara{at}hsr.it

Published 9 December 2015, Sci. Transl. Med. 7, 317ra198 (2015)
DOI: 10.1126/scitranslmed.aac8265

This PDF file includes:

  • Materials and Methods
  • Fig. S1. CD4-CD8 distribution among long-term persisting TK+ cells.
  • Fig. S2. CD95 expression on TK+ cells.
  • Fig. S3. Purification of TK+ cells from T cell subsets.
  • Fig. S4. Functionality of TK suicide gene in long-term persisting gene-modified T cells.
  • Fig. S5. TCR gene usage among gene-modified T cells.
  • Fig. S6. TK+ cell kinetics and persistence after GCV treatment.
  • Fig. S7. Long-term persistence of TK+ cells correlates with early in vivo expansion.
  • Fig. S8. Phenotype of infused TK+ cells in relation to their in vivo expansion.
  • Fig. S9. Phenotype of infused cells in relation to long-term persistence.
  • Fig. S10. Phenotype of infused cells in relation to TK+ cell AUC.
  • Fig. S11. Tracking CMV-specific T cells by dextramers in TK patients.
  • Fig. S12. Tracking Flu-specific T cells by dextramers in TK patients.
  • Fig. S13. Clonal distribution of TK+ cell clones after ex vivo manipulation and purification.
  • Fig. S14. Memory phenotype of TK+ cell clones after ex vivo manipulation and purification.
  • Fig. S15. Gene mapping of retroviral IS retrieved in long-term persisting dominant TK+ cell clones.
  • Fig. S16. Analysis of the distribution of TK+ cell clones among memory T cell subsets.
  • Fig. S17. Differential gene marking of clones after transduction.
  • Table S1. Characteristics of TK patients.
  • Table S2. Immunological parameters of TK patients.
  • Table S3. Variables affecting long-term immune reconstitution after haploidentical HSCT.
  • Table S4. Quantification and isolation of long-term persisting TK+ cells.
  • Table S5. Proliferation of TK+ and TK cells in TK patients.
  • Table S6. CD4-CD8 distribution among TK+ and TK cells.
  • Table S7. Phenotypic characterization of TK+ and TK cells circulating in TK patients.
  • Table S8. Number of unique sequences of TK+ cells.

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