Supplementary Materials

Supplementary Material for:

RANKL blockade prevents and treats aggressive osteosarcomas

Yan Chen, Marco A. Di Grappa, Sam D. Molyneux, Trevor D. McKee, Paul Waterhouse, Josef M. Penninger, Rama Khokha*

*Corresponding author. E-mail: rkhokha{at}

Published 9 December 2015, Sci. Transl. Med. 7, 317ra197 (2015)
DOI: 10.1126/scitranslmed.aad0295

This PDF file includes:

  • Materials and Methods
  • Fig. S1. The kinetics of primary osseous tumor growth from Rb/p53 GEMMs.
  • Fig. S2. OS histology and semiquantitative PCR for Prkar1α alleles.
  • Fig. S3. Compiled analyses for CREB, OPG, and RANK signaling molecules from Rb/p53 GEMMs.
  • Fig. S4. H&E staining of a mandibular OS in one MOTO-Rankl−⁄− mouse.
  • Fig. S5. MTT assay for tumor cells from Rb/p53 GEMMs.
  • Fig. S6. RANK/TRAP staining and qRT-PCR for Opg and Rankl/Opg ratio from MOTO-RankΔ/ΔOC GEMM.
  • Fig. S7. Western blot and densitometry for RANK, NF-κB, and PTEN in MOTO-RankΔ/ΔOC GEMM.
  • Fig. S8. Experimental and spontaneous lung metastasis.
  • Fig. S9. ELISA for serum RANKL concentration.
  • Fig. S10. Body weight of Rb+/ΔOBp53+/ΔOBPrkar1α+/ΔOB mice.
  • Legend for table S1
  • Table S2. Antibodies used for Western blots.
  • References (5055)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 Animals used in this study (provided as a separate Excel file).