Supplementary Materials

Supplementary Material for:

A cocktail of humanized anti–pertussis toxin antibodies limits disease in murine and baboon models of whooping cough

Annalee W. Nguyen, Ellen K. Wagner, Joshua R. Laber, Laura L. Goodfield, William E. Smallridge, Eric T. Harvill, James F. Papin, Roman F. Wolf, Eduardo A. Padlan, Andy Bristol, Michael Kaleko,* Jennifer A. Maynard*

*Corresponding author. E-mail: maynard{at}che.utexas.edu (J.A.M.); mkaleko{at}syntheticbiologics.com (M.K.)

Published 2 December 2015, Sci. Transl. Med. 7, 316ra195 (2015)
DOI: 10.1126/scitranslmed.aad0966

This PDF file includes:

  • Fig. S1. Specific activity of humanized antibody variants.
  • Fig. S2. Antibody thermal stability.
  • Fig. S3. Competition ELISA to assess solution binding affinities of purified antibodies.
  • Fig. S4. Binding kinetics of antibody-PTx interactions.
  • Fig. S5. Pilot murine protection data with recent human clinical isolate D420 and m1B7.
  • Fig. S6. Concentrations of anti-PTx antibodies in baboons.
  • Fig. S7. Detection of the hu1B7-hu11E6 combination in the nasopharyngeal wash of baboons.
  • Fig. S8. Histopathological analysis of lung tissue.
  • Table S1. Humanized antibodies are more similar to the human repertoire than the original murine antibodies.
  • Table S2. Baboon model challenge details.
  • Table S3. Tabulated mouse challenge data.
  • Table S4. Tabulated baboon challenge data.

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