Supplementary Materials

Supplementary Material for:

Tissue transcriptome-driven identification of epidermal growth factor as a chronic kidney disease biomarker

Wenjun Ju,* Viji Nair, Shahaan Smith, Li Zhu, Kerby Shedden, Peter X. K. Song, Laura H. Mariani, Felix H. Eichinger, Celine C. Berthier, Ann Randolph, Jennifer Yi-Chun Lai, Yan Zhou, Jennifer J. Hawkins, Markus Bitzer, Matthew G. Sampson, Martina Thier, Corinne Solier, Gonzalo C. Duran-Pacheco, Guillemette Duchateau-Nguyen, Laurent Essioux, Brigitte Schott, Ivan Formentini, Maria C. Magnone, Maria Bobadilla, Clemens D. Cohen, Serena M. Bagnasco, Laura Barisoni, Jicheng Lv, Hong Zhang, Hai-Yan Wang, Frank C. Brosius, Crystal A. Gadegbeku, Matthias Kretzler,* for the ERCB, C-PROBE, NEPTUNE, and PKU-IgAN Consortium

*Corresponding author. E-mail: wenjunj{at}med.umich.edu (W.J.); kretzler{at}umich.edu (M.K.)

Published 2 December 2015, Sci. Transl. Med. 7, 316ra193 (2015)
DOI: 10.1126/scitranslmed.aac7071

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Baseline eGFR prediction by a three-marker panel in C-PROBE.
  • Fig. S2. EGF and two other candidate mRNAs’ expression patterns in a panel of 84 human organs, tissues, and cell lines.
  • Fig. S3. Correlation of EGF mRNA and uEGF/Cr with eGFR and eGFR slope in DN patients and CKD patients with diabetes.
  • Fig. S4. Correlation of uEGF/Cr with ACR.
  • Fig. S5. EGF as the top upstream regulator of genes correlated with eGFR slope.
  • Fig. S6. Correlation of uEGF/Cr with eGFR slope.
  • Fig. S7. ROC curve and corresponding AUC statistics for models with and without uEGF.
  • Fig. S8. mRNA localization by in situ hybridization: Negative and positive control images.
  • Table S1. Significantly enriched canonical pathways in intrarenal marker set.
  • Table S2. qRT-PCR assays used to validate expression of the intrarenal transcripts.
  • Table S3. Correlations of identified intrarenal transcripts with log2 eGFR of patients from the discovery and two validation cohorts.
  • Table S4. Demographic characteristics of NEPTUNE patients with intrarenal EGF expression data available.
  • Table S5. Top 10 upstream regulators of transcripts correlated with eGFR change over time (eGFR slope).
  • References (4553)

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