Supplementary Materials

Supplementary Material for:

Immune history profoundly affects broadly protective B cell responses to influenza

Sarah F. Andrews, Yunping Huang, Kaval Kaur, Lyubov I. Popova, Irvin Y. Ho, Noel T. Pauli, Carole J. Henry Dunand, William M. Taylor, Samuel Lim, Min Huang, Xinyan Qu, Jane-Hwei Lee, Marlene Salgado-Ferrer, Florian Krammer, Peter Palese, Jens Wrammert, Rafi Ahmed, Patrick C. Wilson*

*Corresponding author. E-mail: wilsonp{at}

Published 2 December 2015, Sci. Transl. Med. 7, 316ra192 (2015)
DOI: 10.1126/scitranslmed.aad0522

This PDF file includes:

  • Fig. S1. Molecular characterization of clonal expansions in consecutive years.
  • Fig. S2. Influenza HA phylogenetic tree.
  • Fig. S3. Heat map graph of individual mAb binding to H1N1 strains.
  • Fig. S4. Comparison of 2009–2010 and 2010–2011 vaccine response.
  • Fig. S5. Detailed serological binding to H1N1 strains of each subject.
  • Fig. S6. Properties of noncompeting HA stalk–binding mAbs.
  • Fig. S7. Molecular characteristics of VH genes coding for HA stalk– versus head-binding mAbs.
  • Fig. S8. Polyreactivity of HA stalk–binding mAbs.
  • Table S1. Demographic information for subjects described in Fig. 1.
  • Table S2. Demographic information of subjects described in Figs. 2 to 5.
  • Table S3. Sequence information and functional characteristics of all mAbs described in Figs. 2 to 7.
  • Table S4. List of mAbs used in Figs. 6 and 7.

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Other Supplementary Material for this manuscript includes the following:

  • Data S1 Excel file with source data for figures with n < 20.