Supplementary Materials

Supplementary Material for:

Bioengineered vocal fold mucosa for voice restoration

Changying Ling, Qiyao Li, Matthew E. Brown, Yo Kishimoto, Yutaka Toya, Erin E. Devine, Kyeong-Ok Choi, Kohei Nishimoto, Ian G. Norman, Tenzin Tsegyal, Jack J. Jiang, William J. Burlingham, Sundaram Gunasekaran, Lloyd M. Smith, Brian L. Frey, Nathan V. Welham*

*Corresponding author. E-mail: welham{at}

Published 18 November 2015, Sci. Transl. Med. 7, 314ra187 (2015)
DOI: 10.1126/scitranslmed.aab4014

This PDF file includes:

  • Methods
  • Fig. S1. Explant culture of primary human VF mucosal cells.
  • Fig. S2. VFF distribution and contractile function in type I collagen scaffold.
  • Fig. S3. Assembly of engineered VF mucosa.
  • Fig. S4. Additional histologic characterization of engineered and native human VF lamina propria.
  • Fig. S5. Enlargement of enriched ontology terms within the engineered VF mucosa proteome.
  • Fig. S6. Additional quantitative proteomic analysis of engineered VF mucosa compared to its isolated subcomponents.
  • Fig. S7. Ex vivo setup used to test physiologic performance of engineered VF mucosa.
  • Fig. S8. Experimental approach used to evaluate in vivo immunogenicity.
  • Fig. S9. Additional in vivo graft survival and immunogenicity data.
  • Table S1. Human donor demographic, surgical, and diagnostic information.
  • Table S2. Lists of proteins identified in LC-MS/MS analysis of scaffold only, VFF in scaffold, VFE on scaffold, engineered VF mucosa, and native VF mucosa.
  • Table S3. Gene ontology (biological process, cellular component, and molecular function) terms enriched in the engineered VF mucosa proteome.
  • Table S4. Lists of ECM and organogenesis/morphogenesis proteins identified in both engineered and native VF mucosae.
  • Table S5. Lists of proteins significantly overrepresented in engineered VF mucosa compared to VFE on scaffold, VFE on scaffold compared to engineered VF mucosa, engineered VF mucosa compared to VFF in scaffold, and VFF in scaffold compared to engineered VF mucosa.
  • Table S6. Gene ontology biological process terms enriched in the protein set exclusive to engineered VF mucosa or overrepresented in engineered mucosa compared to both VFF in scaffold and VFE on scaffold.
  • Table S7. Antibodies and isotype controls used for flow cytometry.
  • Table S8. Antibodies used for immunocytochemistry and immunohistochemistry.
  • Legends for movies S1 and S2
  • References (3640)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Physiologic vibratory function in a single larynx after unilateral placement of engineered VF mucosa.
  • Movie S2 (.mp4 format). Representative acoustic output from a single larynx.