Supplementary Materials

Supplementary Material for:

IL-2Rα mediates temporal regulation of IL-2 signaling and enhances immunotherapy

Ee W. Su, Caitlin J. Moore, Samantha Suriano, Christopher Bryce Johnson, Neizel Songalia, Alicia Patterson, Daniel J. Neitzke, Kristina Andrijauskaite, Elizabeth Garrett-Mayer, Shikhar Mehrotra, Chrystal M. Paulos, Andrew L. Doedens, Ananda W. Goldrath, Zihai Li, David J. Cole, Mark P. Rubinstein*

*Corresponding author. E-mail: markrubinstein{at}musc.edu

Published 28 October 2015, Sci. Transl. Med. 7, 311ra170 (2015)
DOI: 10.1126/scitranslmed.aac8155

This PDF file includes:

  • Fig. S1. IL-2/mAb complexes selectively enhance the persistence of donor T cells.
  • Fig. S2. In the absence of donor T cells, hIL-2/mAb and IL-15/sIL-15Rα complexes mediate comparable antitumor immunity.
  • Fig. S3. Treatment with IL-2/mAb, IL-2/mAbCD25, and IL-15/sIL-15Rα complexes induces differential expansion of CD8+ memory-phenotype T cells, NK cells, and T regulatory cells.
  • Fig. S4. Tc1 but not Tc0 effector CD8+ T cells show preferential responsiveness to IL-2/mAb complexes.
  • Fig. S5. Blockade of IL-2Rα has minimal impact on Tc1 cells in response to titrated IL-2 or IL-15.
  • Fig. S6. Tc1 effector CD8+ T cells exhibit comparable functional sensitivity to IL-2 and IL-15 in vitro.
  • Fig. S7. Tc1 effector CD8+ T cells pulsed with IL-2 mediate sustained cytokine signaling.
  • Fig. S8. IL-2–mediated sustained cytokine signaling is IL-2Rα–dependent in 11 independent experiments.
  • Fig. S9. Tc1 effector CD8+ T cells pulsed with IL-2 exhibit IL-2Rα–dependent proliferation after cytokine withdrawal.
  • Fig. S10. Human IL-2 mediates sustained cytokine signaling on mouse Tc1 effector CD8+ T cells.
  • Fig. S11. Human effector CD8+ T cells pulsed with IL-2 mediate sustained IL-2Rα–dependent signaling.
  • Fig. S12. Human IL-2/mAb (clone 5355), but not mouse IL-2/mAbCD122 (clone S4B6), complexes are permissive to IL-2Rα–dependent sustainedsignaling in vitro.
  • Fig. S13. Antibodies for mouse and human IL-2 are species-specific.
  • Fig. S14. Detection of hIL-2 by confocal microscopy is species-specific and dependent on pulsing cells with cytokine at 37°C.
  • Fig. S15. Detection of mIL-2 by confocal microscopy.
  • Fig. S16. Colocalization of hIL-2 with EEA1 and LAMP-1 by confocal microscopy.
  • Fig. S17. In lymphodepleted mice, IL-15/sIL-15Rα and hIL-2/mAb mediate comparable engraftment of Tc1 effector CD8+ T cells.

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data for main and supplementary figures.