Supplementary Materials

Supplementary Material for:

Identification of broadly conserved cross-species protective Leishmania antigen and its responding CD4+ T cells

Zhirong Mou, Jintao Li, Thouraya Boussoffara, Hiroyuki Kishi, Hiroshi Hamana, Peyman Ezzati, Chuanmin Hu, Weijing Yi, Dong Liu, Forough Khadem, Ifeoma Okwor, Ping Jia, Kiyomi Shitaoka, Shufeng Wang, Momar Ndao, Christine Petersen, Jianping Chen, Sima Rafati, Hechmi Louzir, Atsushi Muraguchi, John A. Wilkins, Jude E. Uzonna*

*Corresponding author. E-mail: jude.uzonna{at}umanitoba.ca

Published 21 October 2015, Sci. Transl. Med. 7, 310ra167 (2015)
DOI: 10.1126/scitranslmed.aac5477

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Bone marrow cells from C57BL/6 mice were differentiated to dendritic cells (BMDCs) with GM-CSF in vitro and infected with L. major promastigotes.
  • Fig. S2. Computer models showing binding of P1 (upper panel) and P3 (PEPCK335–351, lower panel) to MHC class II (I-Ab) molecule and their respective binding energies.
  • Fig. S3. Multiple amino acid sequence alignment of PEPCK from various Leishmania genus.
  • Fig. S4. Comparison of amino acid sequences of Leishmania-specific TCRα chain.
  • Fig. S5. Direct ex vivo cytokine profile of PEPCK335–351–specific CD4+ T cells from the spleens of L. major–infected mice.
  • Fig. S6. Expression of memory markers on non–PEPCK335–351–specific CD4+ T cells from L. major–infected mice.
  • Fig. S7. Prechallenge immune response in the draining LNs and spleens in mice immunized with PEPCK335–351 (P3) peptide.
  • Fig. S8. Postchallenge immune response in dLNs of mice immunized with the PEPCK335–351 (P3) peptide.
  • Fig. S9. Antibody responses against PEPCK in different hosts infected with Leishmania.
  • Table S1. Amino acid sequences and the corresponding source proteins of some key peptides identified by both GPM and ProteinPilot software.
  • Table S2. DNA sequence of primers and artificial DNAs.
  • Source data
  • References (4244)

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